亲水色谱在食品内源组分分离分析方面的应用进展

Progress of Application of Hydrophilic Interaction Liquid Chromatography in the Analysis of Food Endogenous Components Separation

亲水色谱是一种类似于正相色谱保留模式的分离手段,主要以水(2%~40%)和乙腈(大于60%)作为流动相在极性固定相表面完成目标物质分离。在亲水色谱分离模式下,化合物按照极性由弱到强依次出峰,因此其可作为分析强极性化合物和离子化合物的适宜手段。近些年亲水色谱在食品科学方面的应用越来越广泛。亲水色谱在食品分离分析方面的应用对象已包含食品内源组分、食品外源污染物和毒性物质等。本文主要针对亲水色谱在食品内源组分(核苷碱基、核苷、核苷酸,氨基酸、多肽、蛋白质,磷脂酰胆碱类化合物,碳水化合物,维生素等物质)的应用展开讨论,以期为食品中未知成分的分离分析提供参考。

Hydrophilic interaction liquid chromatography(HILIC)is a separation method similar to the retention mode of normal phase chromatography,which combined of a polar stationary phase(SP)and a mobile phase(MP)containing 2%~40%of water and acetonitrile(>60%)for the separation of target substances.HILIC is a suitable approach for the separation of strong polar compounds and ionic compounds due to its compounds elution order according to polarity from weak to strong.Nowdays,HILIC has been widely used in food science,the application objects contains endogenous components,exogenous contaminants and toxic compounds.The paper mainly focuses on the application of hydrophilic chromatography in endogenous components of food,including nucleobases,nucleosides,nucleotides,amino acids,peptides,proteins,choline-related compounds,phosphatidylcholine compounds,carbohydrates and vitamins,in order to provide a reference for the separation analysis of unknown components in food.