肺癌组织中磷脂酰肌醇3激酶、Notch-1水平与预后关系

Relationships between phosphatidylinositol 3-kinase,Notch-1 levels and prognosis in patients with lung cancer

目的探讨肺癌病人组织中磷脂酰肌醇3激酶(PI3K)、Notch-1水平与预后的关系。方法选取2012年5月至2014年5月于商洛市中心医院行全胸腔镜肺叶切除术治疗的肺癌病人60例为研究对象,取其癌组织及癌旁组织,采用免疫组化法检测癌组织及癌旁组织中PI3K、Notch-1蛋白阳性表达水平,通过单因素和多因素分析比较PI3K、Notch-1水平与病人各临床病理特征及预后的关系。结果癌组织与癌旁组织PI3K蛋白表达阳性率分别为81.67%和5.00%,Notch-1蛋白表达阳性率分别为65.00%和8.33%,两者在癌组织中的表达均显著高于癌旁组织(P<0.05);且PI3K、Notch-1蛋白表达水平与临床TNM分期、分化程度、淋巴结转移有关(P<0.05),而与病人的性别、年龄、吸烟、饮酒及病理分型情况无关(P>0.05);Kaplan-Meier分析结果表明:PI3K、Notch-1蛋白高表达者中位生存时间短于低表达者(P<0.05);logistic多因素分析显示影响肺癌病人术后预后的独立危险因素为TNM分期和PI3K。结论肺癌病人组织中PI3K、Notch-1蛋白高表达,TNM分期和PI3K可影响病人预后。

Objective To investigate the relationships between phosphatidylinositol 3-kinase(PI3K),Notch-1 levels and prognosis in patients with lung cancer.Methods Sixty patients with lung cancer who underwent total thoracoscopic lobectomy in Shangluo Central Hospital from May 2012 to May 2014 were selected as the study subjects.The cancer tissues and adjacent tissues were taken,the expressions of PI3K and Notch-1 proteins in cancer tissues and adjacent tissues were detected by immunohistochemistry,and the relationships between PI3K,Notch-1 levels and clinicopathological features,prognosis were compared by univariate and multivariate analysis.Results The positive rates of PI3K protein expression in cancer tissues and adjacent tissues were 81.67%and 5.00%,respectively,the positive rates of Notch-1 protein expression were 65.00%and 8.33%respectively,both of them were significantly higher in cancer tissues than in adjacent tissues(P<0.05);the expression levels of PI3K and Notch-1 proteins were correlated with clinical TNM stage,differentiation degree and lymph node metastasis(P<0.05),but not with gender,age,smoking,drinking and pathological classification(P>0.05);Kaplan-Meier analysis showed that the median survival time of PI3K and Notch-1 proteins high expression was shorter than that of low expression(P<0.05);logistic multivariate analysis showed that TNM stage and PI3K were independent risk factors affecting the prognosis of lung cancer patients.Conclusion The expressions of PI3K and Notch-1 proteins are highly expression in lung cancer patients,TNM stage and PI3K can affect the prognosis of patients.