肉芽肿小叶性乳腺炎大鼠模型建立初探

Establishment of a Rat Model of Granulomatous Lobular Mastitis

目的利用人肉芽肿小叶性乳腺炎标本匀浆和完全弗氏佐剂的混悬液构建肉芽肿小叶性乳腺炎动物模型。方法将25只有孕产史的SD大鼠随机分为5组:空白对照组、完全弗氏佐剂组、正常乳腺组织匀浆组、病变乳腺组织匀浆低剂量组和病变乳腺组织匀浆高剂量组,每组5只。病变乳腺组织匀浆组以人肉芽肿性乳腺炎组织匀浆制备的完全弗氏佐剂混悬液,接种于大鼠第3、4对乳腺组织(高剂量组注射0.2 mL,低剂量组注射0.1 mL);正常乳腺组织匀浆组接种肉芽肿性乳腺炎患者的正常乳腺组织匀浆液;空白对照组接种生理盐水(即0.9%NaCl溶液);完全弗氏佐剂组接种完全弗氏佐剂。造模完成后维持饲养14 d,观察组织形态学及HE切片染色后的组织病理学特征,比较肉芽肿小叶性乳腺炎模型的建立效果。结果与空白对照组、完全弗氏佐剂组、正常乳腺组织匀浆组对比,病变乳腺组织匀浆高低剂量组均呈现不同程度的肉芽肿小叶性乳腺炎特有的形态学改变及病理学特征,组间对比差异具有统计学意义(P<0.001)。结论人肉芽肿小叶性乳腺炎标本匀浆和完全弗氏佐剂的混悬液可成功建立肉芽肿小叶性乳腺炎大鼠模型,为该病的病因学研究奠定了基础。

Objective In this study,an animal model of granulomatous lobular mastitis was constructed using human granulomatous lobular mastitis specimen homogenates and a suspension of complete Freund's adjuvant.Methods Twenty-five rats with a history of maternity were randomly divided into five groups with five rats each:a blank control group,Freund's adjuvant group,normal mammary homogenate group,low-dose lesion homogenate group,and high-dose lesion homogenate group.In the lesion homogenization groups,rats were injected with complete Freund's adjuvant homogenate prepared from granulomatous mastitis patient tissues,and the third and fourth pair of mammary gland injections were administered to the rats at 0.2 mL in the high-dose group and 0.1 mL in the low-dose group.The normal mammary gland homogenate group was injected with normal mammary gland tissue homogenate from patients with granulomatous mastitis.The blank control group was injected with saline.The Freund's adjuvant group was injected with complete Freund's adjuvant.The histomorphological and pathophysiological characteristics of the mammary tissues were observed for 14 days after the completion of modeling,and the effects of the granulomatous lobular mastitis model were compared.Results The morphological changes and physiopathological characteristics of granulomatous lobular mastitis in the high-and low-dose groups were significantly different when compared with the blank control,Freund's adjuvant,and normal breast homogenate groups(P<0.001).Conclusion Human granulomatous lobular mastitis specimen homogenates and suspensions of complete Freund's adjuvant for granulomatous lobular mastitis can successfully establish an animal model of the disease and lead to insights and new ideas for the etiology of the disease.