外周血miR-34a、SIRT1水平与脑梗死后认知功能障碍的关系

The Relationships between the Levels of miR-34a,SIRT1 in Peripheral Blood and Cognitive Dysfunction after Cerebral Infarction

目的探讨外周血微小RNA-34a(miR-34a)、沉默信息调节因子1(SIRT1)水平与脑梗死后认知功能障碍的关系。方法选取2017年9月—2019年11月我院收治的脑梗死后认知功能障碍病人145例为研究对象(观察组),同期脑梗死未发生认知功能障碍病人145例作为对照组。入院后次日抽取所有病人外周静脉血,分离血清,采用实时荧光定量逆转录聚合酶链式反应(qRT-PCR)检测血清miR-34a、SIRT1水平;采用简易智能精神状态检查量表(MMSE)、蒙特利尔认知评估量表(MoCA)评估病人认知功能;采用Pearson法分析脑梗死后认知功能障碍病人血清miR-34a、SIRT1水平与MMSE评分、MoCA评分的相关性;采用多因素Logistic回归分析影响脑梗死病人发生认知功能障碍的因素。结果与对照组比较,观察组血清miR-34a水平升高(P<0.05),血清SIRT1水平、MMSE评分、MoCA评分降低(P<0.05)。血清miR-34a水平是影响脑梗死病人发生认知功能障碍的独立危险因素(P<0.05),血清SIRT1水平是保护因素(P<0.05);脑梗死后认知功能障碍病人血清miR-34a水平与MMSE评分、MoCA评分、SIRT1水平均呈负相关(P<0.05),血清SIRT1水平与MMSE评分、MoCA评分均呈正相关(P<0.05)。结论脑梗死后认知功能障碍病人外周血miR-34a呈高表达、SIRT1呈低表达,二者可能共同参与认知功能障碍的发生过程。

Objective To investigate the relationships between the levels of microRNA-34a(miR-34a),silent information regulator 1(SIRT1)in peripheral blood and cognitive dysfunction after cerebral infarction.Methods One hundred and forty-five patients with cognitive dysfunction after cerebral infarction were selected as the observation group,and 145 patients without cognitive dysfunction after cerebral infarction during the same period were selected as the control group.Peripheral venous blood was collected from all patients on the second day after admission,and serum was separated.The levels of miR-34a and SIRT1 were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR).The cognitive function of the patients was assessed by Simple Mental State Examination Scale(MMSE)and Montreal Cognitive Assessment Scale(MoCA).The correlations between the levels of serum miR-34a,SIRT1 and MMSE score,MoCA score were analyzed by Pearson′s method.The influencing factors of cognitive dysfunction in patients with cerebral infarction were analyzed by multivariate Logistic regression.Results Compared with the control group,the level of serum miR-34a in the observation group was significantly increased(P<0.05),the level of serum SIRT1,MMSE score and MoCA score was significantly decreased(P<0.05).The levels of serum miR-34a was an independent risk factor for cognitive dysfunction in patients with cerebral infarction.The levels of serum SIRT1 was a protective factor(P<0.05).There was significant negative correlation between the level serum of miR-34a and MMSE score,MoCA score,SIRT1 level(P<0.05),and the level of serum SIRT1 was positively correlated with MMSE score and MoCA score(P<0.05).Conclusion miR-34a was highly expressed and SIRT1 was low expressed in peripheral blood of patients with cognitive dysfunction after cerebral infarction,which might beinvolved in the occurrence of cognitive dysfunction together.