解郁养肝汤对抑郁大鼠海马及血清IL-1β、IL-6的影响

Effects of Jieyu Yanggan Decoction on IL-1βand IL-6 in Hippocampus and Serum of Depressed Rats

目的观察解郁养肝汤对抑郁模型大鼠海马及血清炎性因子白细胞介素1β(IL-1β)、白细胞介素6(IL-6)水平的影响。方法将成年SD雌性大鼠140只分为空白对照组、模型组、氟西汀组、解郁颗粒组、解郁养肝汤高剂量组、解郁养肝汤中剂量组和解郁养肝汤低剂量组。采用慢性不可预知应激刺激与孤养结合的方法造成抑郁模型,连续给药3周后,检测大鼠海马及血清IL-1β、IL-6水平。结果解郁养肝汤高剂量组、解郁养肝汤中剂量组和解郁养肝汤低剂量组大鼠血清及海马IL-1β、IL-6含量较盐酸氟西汀组、解郁颗粒组降低(P<0.05或P<0.01)。结论解郁养肝汤可改善抑郁模型大鼠抑郁状态和疲乏症状,其作用机制可能是通过下调机体炎性因子IL-1β、IL-6含量实现的。

Objective To observe the effect of Jieyu Yanggan Decoction on interleukin 1β(IL-1β)and interleukin 6(IL-6)in hippocampus and serum of depression model rats.Methods One hundred and forty adult SD rats were divided into blank control group,model group,fluoxetine group,Jieyu granule group,Jieyu Yangan Decoction high-dose group,Jieyu Yangan Decoction medium-dose group and Jieyu Yangan Decoction low-dose group.The depression model was created by the combination of chronic unpredictable stress and isolation.After 3 weeks of continuous administration,the levels of IL-1βand IL-6 in hippocampus and serum were detected.Results Compared with the fluoxetine group and Jieyu granule group,the levels of IL-1βand IL-6 in serum and hippocampus of Jieyu Yangan Decoction high-dose group,Jieyu Yangan Decoction medium-dose group and Jieyu Yangan Decoction low-dose group were alldecreased(P<0.05or P<0.01).Conclusion Jieyu Yanggan Decoction could significantly improve the depression and fatigue symptoms of experimental rats with depression.The mechanism of action might be achieved by down-regulating the levels of IL-1βand IL-6.