三七叶水提物对5-氟尿嘧啶诱导小鼠肠道黏膜炎的预防作用

Preventive Effect of an Aqueous Extract from Leaves of Panax notoginseng on 5-Fluorouracil-Induced Intestinal Mucositis in Mice

探究三七叶水提物对5-氟尿嘧啶(5-fluorouracil,5-FU)所致肠道黏膜炎的预防作用。选用昆明雄性小鼠,各组通过灌胃给药,然后腹腔注射5-FU建立肠道黏膜炎模型。观察小鼠体重变化情况,采用HE染色观察空肠组织形态学变化,免疫组织化学法观察空肠增殖细胞核抗原(PCNA)表达,实时荧光定量聚合酶链式反应测定空肠屏障相关基因黏蛋白2(MUC-2)、闭锁小带蛋白1(ZO-1)、闭合蛋白(Occludin)和炎症相关基因白介素1β(IL-1β)、白介素6(IL-6)、肿瘤坏死因子α(TNF-α)mRNA的表达量。与模型组相比,三七叶水提物高剂量组小鼠体重升高了49.24%,高剂量组空肠绒毛长度升高了22.92%、隐窝深度降低了16.27%、绒毛长度与隐窝深度比值升高了42.32%,高剂量组空肠PCNA表达量上升了21.54%,高剂量组空肠MUC-2、ZO-1、Occludin mRNA表达量分别升高了157.66%、167.73%、212.14%;高剂量组空肠IL-1β、IL-6、TNF-αmRNA表达量分别降低了34.24%、35.18%、33.52%。以上结果表明,三七叶水提物可以预防5-FU引起的肠道黏膜炎,其能力呈现浓度依赖。

The objective of this study was to explore the preventive effect of an aqueous extract of Panax notoginseng leaves on 5-fluorouracil(5-FU)-induced intestinal mucositis.Kunming male mice were selected.Each group was administered intragastrically before 5-FU was injected intraperitoneally to establish an intestinal mucositis model.The changes in the body weights of mice were recorded.The morphological changes of the jejunum were examined by HE staining.The expression of proliferating cell nuclear antigen(PCNA)in the jejunum was examined by immunohistochemistry.The mRNA expressions of MUC2,ZO-1,Occludin,IL-1β,IL-6 and TNF-αwere determined by real-time fluorescence quantitative polymerase chain reaction.Compared with the model group,the body weights of the mice in the high-dose group increased by 49.24%,and their jejunum villus length increased by 22.92%,crypt depth decreased by 16.27%,villus length-to-crypt depth ratio increased by 42.32%,and expression of PCNA in the jejunum increased by 21.54%.The mRNA expressions of MUC-2, ZO-1 and Occludin in jejunum of high-dose group increased by 157.66%, 167.73% and 212.14% respectively, the mRNA expression of IL-1β, IL-6 and TNF-α in the jejunum of the high-dose group decreased by 34.24%, 35.18% and 33.52% respectively. The above-described results showed that the aqueous extract of Panax notoginseng leaves could prevent 5-FU-induced intestinal mucositis in a concentration-dependent manner.