网笼饲养对大鼠生理指标的影响

The Effect of Cage Feeding on the Physiological Indexes of Rats

目的探讨网笼饲养对SD大鼠生理指标的影响。方法自上海斯莱克实验动物有限公司采购40只(20♂20♀)SD大鼠,SPF级,随机分为网笼组和玉米芯垫料组,每组20只(10♂10♀),网笼组使用网笼饲养,玉米芯垫料组使用玉米芯垫料饲养,饲养周期为10周,每周称量体重1次。于第10周解剖检查,对主要脏器(心、肝、脾、肾)进行肉眼观察及质量测定;采集血液、尿液,测定血液学(WBC、RBC、HGB、HCT、MCV、MCH、MCHC)、血液生化学(ALT、AST、BUN、CREA)、尿液(PRO、GLU、KET、LEU、ERY、UBG、BIL),比较两组上述指标的差异。结果①体重:实验第1周,网笼组雄性大鼠体重低于玉米芯垫料组,差异有统计学意义(P<0.05);第2~10周,两组体重比较,差异无统计学意义(P>0.05);②脏器质量及指数:网笼组雄性大鼠肝脏质量低于玉米芯垫料组,差异有统计学意义(P<0.05),两组其他脏器质量及系数比较,差异无统计学意义(P>0.05);③血液学:网笼组雄性大鼠RBC高于玉米芯垫料组,差异有统计学意义(P<0.05),两组其他血液学指标比较,差异无统计学意义(P>0.05);④血液生化:网笼组雄性大鼠ALT、CREA低于玉米芯垫料组,差异有统计学意义(P<0.05)。两组其他生化指标比较,差异无统计学意义(P>0.05)。结论网笼饲养与玉米芯垫料饲养均能保证大鼠的正常生长,未对大鼠生理指标造成明显影响。

Objective To investigate the effect of cage feeding on the physiological indexes of SD rats.Methods 40(20♂20♀)SD rats were purchased from Shanghai Slack Laboratory Animal Co.,Ltd.,SPF grade,and randomly divided into net cage group and corncob litter group,20 rats in each group(10♂10♀).The net cage group was reared in net cages,and the corncob litter group was reared with corncob litter.The feeding period was 10 weeks,and the body weight was weighed once a week.At the 10th week,an autopsy was performed,and the main organs(heart,liver,spleen,kidney)were visually observed and the quality was measured;Collect blood and urine,measure hematology(WBC,RBC,HGB,HCT,MCV,MCH,MCHC),blood biochemistry(ALT,AST,BUN,CREA),urine(PRO,GLU,KET,LEU,ERY,UBG,BIL),compare the differences of the above indicators between the two groups.Results①Body weight:In the first week of the experiment,the weight of male rats in the cage group was lower than that in the corncob litter group,the difference was statistically significant(P<0.05);From 2 to 10 weeks,there was no significant difference in body weight between the two groups(P>0.05);②Organ quality and index:The liver quality of male rats in the cage group was lower than that in the corncob litter group,the difference was statistically significant(P<0.05).There was no statistically significant difference in the quality and coefficient of other organs between the two groups(P>0.05);③Hematology:The RBC of male rats in the cage group was higher than that in the corncob bedding group,the difference was statistically significant(P<0.05).There was no statistically significant difference in other hematological indicators between the two groups(P>0.05);④Blood biochemistry:The ALT and CREA of male rats in the cage group were lower than those in the corncob litter group,the difference was statistically significant(P<0.05).There was no statistically significant difference in biochemical indicators between the two groups(P>0.05).Conclusion Both cage feeding and corncob feeding can ensure the normal growth of rats,and do not significantly affect the physiological indicators of rats.