红纹凤仙花离体快繁技术体系的建立

Establishment of Rapid Propagation Technology for Impatiens rubrostriata in vitro

以红纹凤仙花(Impatiens rubrostriata Hook.f.)带芽茎段为试材,采用不同灭菌剂对外植体进行处理,通过添加不同种类植物生长调节剂及浓度的培养基对其进行初代培养、增殖及生根培养,从而筛选红纹凤仙花最佳灭菌方法、最佳诱导、增殖及生根培养基,以期为野生凤仙花属植物资源的引种驯化及开发利用奠定良好的技术基础。结果表明:红纹凤仙花带芽茎段的最适灭菌方法为75%酒精10 s+0.1%氯化汞6 min,成活率为68%;初代培养基中添加20 mg·L^(-1)头孢噻肟钠时抑菌效果较好,成活率100%;最适初代培养基为MS+1.0 mg·L^(-1)6-BA+0.3 mg·L^(-1)NAA,苗壮且生长快;最适增殖培养基为MS+1.0 mg·L^(-1)6-BA+0.3 mg·L^(-1)NAA+0.010 mg·L^(-1)TDZ,增殖系数为6.65,苗生长快;最适生根培养基为MS+0.10 mg·L^(-1)IBA,生根率100%。

The budding stems of Impatiens rubrostriata were used as materials.The explants were treated with different sterilants,and primary culture,proliferation and rooting culture.They were performed on the medium with different types of hormoneand concentrations to screen the best sterilization method,induction,proliferation and rooting medium of Impatiens rubrostriata.The results showed that the optimal sterilization method for Impatiens rubrostriatais 75%alcohol,10 seconds+0.1%mercury chloride for 6 minutes,the survival rate was 100%.When 20 mg·L^(-1)cefotaxime sodium was added to the primary medium,the bacterial effect was better,the survival rate was 100%.The most suitable primary medium was MS+1.0 mg·L^(-1)6-BA+0.3 mg·L^(-1)NAA,under this circumstances,the seedlings were strong and the growth was fast.The optimal proliferation medium was MS+1.0 mg·L^(-1)6-BA+0.3 mg·L^(-1)NAA+0.010 mg·L^(-1)TDZ,the proliferation coefficient was 6.65,the seedlings grew fast.The most suitable rooting medium was MS+0.10 mg·L^(-1)IBA,and the rooting rate was 100%.The in vitro rapid propagation system for Impatiens rubrostriata was established,which will lay a good technical foundation for development and utilization of Impatiens rubrostriata.