LncRNA SNHG14调控miR-656-3p/SIRT5通路加重肝癌侵袭和迁移

LncRNA SNHG14 Regulates miR-6-3p/SIRT5 Pathway to Aggravate Liver Cancer Invasion and Migration

目的:探讨长链非编码RNA(lncRNA)小核仁RNA宿主基因14(SNHG14)对肝细胞癌(HCC)细胞侵袭和迁移的影响及机制。方法:采用实时荧光定量聚合酶链式反应(qRT-PCR)检测肝癌细胞SNHG14和miR-656-3p的表达。与sh-SNHG14,miR-656-3p抑制剂,miR-656-3p模拟物,pcDNA3.1-SIRT5共转染后,检测HepG2和MHCC97H细胞增殖、侵袭和迁移。然后用qRT-PCR测定SNHG14、miR-656-3p和SIRT5的表达水平,荧光素酶报告基因检测和RNA下调SNHG14与miR-656-3p之间的关系,以及miR-656-3p与SIRT5之间的关系。结果:HCC细胞中SNHG14表达上调,miR-656-3p表达下调。抑制SNHG14和miR-656-3p过表达,可抑制HepG2和MHCC97H细胞增殖,侵袭和迁移。SNHG14直接作用于miR-656-3p,SIRT5是miR-656-3p的靶基因。miR-656-3p抑制剂或pcDNA3.1-SIRT5可逆转sh-SN HG14对肝癌细胞增殖、侵袭和迁移的抑制作用。结论:SNHG14通过调节miR-656-3p/SIRT5轴促进肝癌细胞的侵袭和迁移。

Objective To investigate the effect and mechanism of long non-coding RNA(lncRNA)small nucleolar RNA host gene 14(SNHG14)on the invasion and migration of hepatocellular carcinoma(HCC)cells.Methods Real-time fluorescent quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of SNHG14 and miR-656-3p in liver cancer cells.After co-transfection with sh–SNHG14,miR–656–3p inhibitor,miR–656–3p mimic,and pcDNA3.1–SIRT5,the proliferation,invasion and migration of HepG2 and MHCC97H cells were detected.Then qRT-PCR was used to determine the expression levels of SNHG14,miR-656-3p and SIRT5,luciferase reporter gene detection and RNA down-regulation between SNHG14 and miR-656-3p,and between miR-656-3p and SIRT5 Relationship.Results The expression of SNHG14 was up-regulated in HCC cells,and the expression of miR-656-3p was down-regulated.Inhibiting the overexpression of SNHG14 and miR-656-3p can inhibit the proliferation,invasion and migration of HepG2 and MHCC97H cells.SNHG14 acts directly on miR-656-3p,and SIRT5 is the target gene of miR-656-3p.miR–656–3p inhibitor or pcDNA3.1–SIRT5 can reverse the inhibitory effects of sh–SN HG14 on the proliferation,invasion and migration of liver cancer cells.Conclusion SNHG14 promotes the invasion and migration of liver cancer cells by regulating the miR–656–3p/SIRT5 axis.