Xpert MTB/RIF技术在结核分枝杆菌快速检测的价值

The Value of Xpert MTB/RIF Technology in Rapid Detection of Mycobacterium Tuberculosis

目的:讨论Xpert MTB/RIF技术对快速检出结核分枝杆菌的意义,以期为临床提供帮助。方法:收集我院854例疑似肺结核患者痰标本,分别用抗酸染色法、Xpert MTB/RIF法和液体培养法进行检测。以综合临床诊断为标准,评估Xpert MTB/RIF的敏感性,及在抗酸染色阴性和抗酸染色阳性中比较XpertMTB/RIF法和液体培养法的阳性检出率。结果:XpertMTB/RIF法、液体培养法和抗酸染色法的敏感性分别为60.11%(217/361)、54.02%(195/361)、29.09%(105/361)。以临床诊断为标准,在确诊肺结核样本中,Xpert MTB/RIF法与液体培养法这两种方法的敏感性相较无统计学意义(χ^(2)=0.927p>0.05),在涂阳样本中XpertMTB/RIF法和液体培养法的检出率相较,差异无统计学意义(χ^(2)=0.631p>0.05),在涂阴样本中Xpert MTB/RIF法和液体培养法的检出率相较,差异有统计学意义(χ^(2)=3.911 p<0.05)。结论:Xpert MTB/RIF技术在结核分枝杆菌快速检测中,敏感性与特异性较好,阳性检出率较高,具有较高的临床应用价值。

Objective: To discuss the significance of Xpert MTB/RIF technology for rapid detection of Mycobacterium tuberculosis, in order to provide clinical help. Methods The sputum specimens of 854 patients with suspected tuberculosis in our hospital were collected and tested by acid-fast staining method, Xpert MTB/RIF method and liquid culture method. Based on comprehensive clinical diagnosis, the sensitivity of Xpert MTB/RIF was evaluated, and the positive detection rate of Xpert MTB/RIF method and liquid culture method was compared between negative acid-fast staining and positive acid-fast staining. Results: The sensitivity of Xpert MTB/RIF method, liquid culture method and acid-fast staining method were 60.11 %(217/361), 54.02 %(195/361), 29.09 %(105/361), respectively. Taking clinical diagnosis as the standard, the sensitivity of Xpert MTB/RIF method and liquid culture method was not statistically significant(χ^(2)=0.927 p>0.05) in the confirmed pulmonary tuberculosis samples. Xpert MTB in smear positive samples Compared with the detection rate of RIF method and liquid culture method, the difference was not statistically significant(χ^(2)=0.631 p>0.05). In smeared samples, the detection rate of Xpert MTB/RIF method and liquid culture method was compared, the difference was There is statistical significance(χ^(2)=3.911 p<0.05). Conclusion: Xpert MTB/RIF technology has good sensitivity and specificity in the rapid detection of Mycobacterium tuberculosis, and the positive detection rate is high. It has high clinical application value.