禽腺病毒12个血清型代表毒株的分子生物学鉴定研究

Molecular Biological Identification of 12 Strains of Fowl Adenovirus

为建立和完善禽腺病毒(FAdV)标准毒株库,针对FAdV不同种的纤突(Fiber)和六邻体(Hexon)序列设计引物,对中国兽医药品监察所保藏的FAdV 12个血清型代表毒株进行PCR扩增和遗传进化分析,完成了对毒株的分型鉴定,再结合同种不同血清型病毒Hexon序列上酶切位点的特征,用酶切法验证了毒株不存在同种中不同血清型之间的交叉污染。试验结合遗传进化分析和酶切法对FAdV 12个血清型毒株进行了系统分型鉴定,明确其遗传背景、血清型信息及纯净性,建立了系统完整的FAdV代表毒株毒种库,为FAdV鉴定及相关生物制品的研发和评价奠定了物质基础。

In order to establish and improve the standard strain library of avian adenovirus(FAdV),primers were designed for Fiber and Hexon sequences of different species of FAdV.PCR amplification and genetic evolution analysis were performed on 12 representative strains of FAdV serotypes stored in China Institute of Veterinary Drug Control,and genotype and identification were completed.Combined with the characteristics of the enzyme cleavage sites on the Hexon sequence of different serotypes,the cross contamination between different serotypes of the same virus strain was verified by enzyme cleavage method.In summary,12 serotypes of FAdV were systematically classified and identified by genetic evolution analysis and enzyme digestion method.The genetic background,serotype information and purity of FAdV serotypes were clarified,and a systematic and complete library of representative strains of FAdV was established,which laid a material foundation for the identification of FAdV and the development and evaluation of related biological products.