反硝化微生物燃料电池脱除低 C/N 废水中氮的研究

Study on nitrogen removal from low C/N wastewater by denitrification microbial fuel cell

从污水处理厂新鲜活性污泥中分离筛选出3种反硝化菌株(M1、M2和M3)。考察了3种菌株的生理特性和脱氮效率,并将其应用于反硝化微生物燃料电池(MFC)构建以实现低C/N废水的脱氮和同步产电。结果表明:菌株M1、M2、M3均为假单胞菌属(Pseudomonas),48 h对低碳源反硝化培养液脱氮率分别达81.27%、76.66%、82.89%;菌株M1、M2、M3构建的反硝化MFC在80 h时对模拟废水(TN约250 mg/L,C/N为2.0)的脱氮率分别为93.75%、88.94%、91.36%。其中M1菌株构建的反硝化MFC电压最高且最早达到稳定。反硝化MFC的最适C/N为2.0,最适pH为8.5~9.5。

Three denitrification strains(M1,M2 and M3)were isolated from fresh activated sludge of a wastewater treatment plant.The physiological characteristics and denitrification performance of the three strains were investigated,then they were applied in denitrification microbial fuel cell(MFC)to achieve the synchronous denitrification of low carbon wastewater and electricity generation.The results showed that strains M1,M2 and M3 were all Pseudomonas.After 48 hours of cultivation,the denitrification rates of strains M1,M2 and M3 in low carbon denitrification medium were 81.27%,76.66%and 82.89%,respectively.The denitrification rates of these strains in simulated wastewater(where TN was about 250 mg/L and C/N was 2.0)were 93.75%,88.94%and 91.36%after 80 hours of treatment.The denitrification MFC using M1 strain achieved the highest voltage by using the shortest time.The C/N and pH of the denitrification MFC were optimized to be 2.0 and 8.5-9.5,respectively.