人MRP4蛋白结构与功能预测

Structure and Function Prediction of MRP4in Homo Sapiens

多药耐药相关蛋白4(multidrug resistance-associated protein 4,MRP4)是一种能够利用ATP水解产生的能量转运多种物质的六重跨膜蛋白。本研究应用Expasy、Jpred4、UniProt-KB、NCBI等公共数据库和在线软件分析其理化性质、蛋白结构、结构域、亚细胞定位、互作蛋白以及功能等信息,为其在多种生理病理过程中的作用与机制研究奠定基础。MRP4蛋白含有1325个氨基酸,相对分子质量约为149526.77,理论等电点为8.41。以α-螺旋为主,占比58.42%。含有2个跨膜结构域和2个ATP结合盒以及8个保守结构域。表观遗传学修饰位点56个,其中磷酸化位点27个,泛素化位点27个,乙酰化位点2个。MRP4蛋白分布于质膜上,具有ATP酶和跨膜转运蛋白活性,参与内外源性物质运输、前列腺素分泌、纤毛组装、信号转导运输等生命过程。本研究系统性梳理并整合了MRP4蛋白结构及功能等信息,为深入研究其在机体生命活动及疾病发生中的作用与机制奠定理论依据。

Multidrug resistance-associated protein 4(MRP4)is a six-fold transmembrane protein which plays an important role in the transportation of a variety of substances using the energy generated by ATP hydrolysis.This study aimed to analyze the physical and chemical properties,protein structure,domains,subcellular localization,as well as interacting proteins of MRP4and to describe its potential role and mechanisms of actions in regulating different physiological and pathological processes using public databases and online software such as ExPASy,JPRED4,UniprotKB and NCBI.MRP4contains 1325amino acids with a molecular weight of 149526.77and a theoretical isoelectric point of 8.41.The secondary structure is mainly composed ofα-helix accounting for 58.42%.It has two transmembrane domains,two highly conserved ATP binding cassettes and four conserved domains.MRP4protein also contains 56different epigenetic modification sites,including 27phosphorylation sites,27ubiquitination sites and 2acetylation sites.The MRP4protein has been found to be widely distributed in the plasma membrane,exhibiting ATPase activity as well as transmembrane transporter function,and involved in the regulation of various biological processes such as internal and external material transport,prostaglandin secretion,ciliary assembly,and signal transduction.In this study,the structure and functions of MRP4protein were systematically analyzed and gathered to lay a theoretical foundation for further study of its potential role and mechanisms in both physiological and pathological conditions.