槐花米中芦丁的抗氧化性及抑菌性研究

Study on Antioxidant Activity and Antibacterial Property of Rutin in Sophora Flower Buds

目的:研究槐花米中芦丁的抗氧化性及抑菌性。方法:以中药槐花米为原料,采用碱溶酸沉法提取其中的芦丁并精制,对精制物进行红外光谱分析;采用清除羟基自由基能力测定来分析研究芦丁的抗氧化性;采用滤纸片法以大肠杆菌和金黄色葡萄球菌为抑菌对象,分析芦丁的抑菌性。结果:芦丁浓度越高抗氧化性越强,芦丁的抗氧化性优于抗坏血酸;芦丁对大肠杆菌和金黄色葡萄球菌均有抑菌作用。结论:芦丁有较好的抗氧化性和抑菌性。

Objective: to study the antioxidant activity and antibacterial property of Rutin in sophora flower buds. Methods: Using sophora flower buds as raw material, Rutin was extracted and refined by Alkali solution and acid precipitation, and the antioxidant activity of Rutin was analyzed by infrared spectroscopy, The antibacterial property of Rutin was analyzed by the filter paper method using Escherichia coli and Staphylococcus aureus as the bacteriostasis objects. Results: The higher the concentration of Rutin, the stronger the antioxidant activity, Rutin has better antioxidant activity than ascorbic acid;Rutin has antibacterial effect on Escherichia coli and Staphylococcus aureus. Conclusion: Rutin has better antioxidant activity and antibacterial property.