三桠苦中几个黄酮苷及构象异构现象研究

Research on the Flavonoid Glycosides from Melicope Pteleifolia and Their Conformational Isomerism

目的:研究三桠苦Melicopepteleifolia的化学成分。方法:三桠苦用80%乙醇渗漉提取,提取物用色谱技术分离,采用波谱技术(ESI-MS、1H-NMR、13C-NMR等)和化学方法进行结构鉴定。结果:从三桠苦乙醇提取物的醋酸乙酯部位分离出4个化合物,分别鉴定为山奈酚-3-O-α-L-阿拉伯吡喃糖苷(1)、山奈酚-3-O-芸香糖苷(2)、异鼠李素-3-O-α-L-阿拉伯吡喃糖苷(3)和山奈酚-3-O-β-D-葡萄吡喃糖苷(4)。结论:所有化合物均为首次从该属植物中分离得到。黄酮苷的构象异构引起核磁共振碳谱信号裂分。

Objective: To study the constituents of Melicope pteleifolia. Methods: Plant material was percolated with 80 % EtOH. Compounds were separated by means of chromatography technology and their structures were elucidated by spectral analysis(ESI-MS,1 H NMR, and 13 C NMR) and chemical evidence. Results: Four flavonoid glycosides were isolated from ethyl acetate soluble fractions. Their structures were established as kaempferol-3-O-α-L-arabinopyranoside(1), kaempferol-3-O-rutinoside(2), isorhamnerin-3-O-α-L-arabinopyranoside(3) and kaempferol-3-O-β-D-glucopyranoside(4). Conclusion: All the compounds are isolated from Melicope genus for the first time. The conformational isomerism of flavonoid glycosides causes the splitting of13 C NMR signal.