白茅根多糖的分离纯化工艺研究

Study on Extraction and Purification of Polysaccharides from Lalang Grass Rhizome

以白茅根为原料,采用超声波辅助热水浸提法提取白茅根多糖,并对其进行分离和纯化。首先对白茅根采用热水超声提取,80%乙醇沉淀后得到白茅根粗多糖P80,提取率为6.81%,苯酚-硫酸法测得P80的糖含量为46.5%。对P80进行Sevag法除去蛋白、透析、冻干等操作,得到LGR80。随后使用DEAE Focurose 6FF阴离子交换柱层析对LGR80进行分离,得到LGR80-1和LGR80-2两个洗脱部位。采用Sephadex G-75凝胶柱分别对LGR80-1和LGR80-2进一步纯化,得到LGRP80-1和LGRP80-2两个多糖成分,用高效凝胶渗透色谱进行纯度检验,结果表明白茅根多糖LGRP80-1和LGRP80-2均具有较高的纯度。

The polysaccharides were extracted from Lalang Grass Rhizome by ultrasonic assisted hot water extraction, and then separated and purified. Firstly,the polysaccharide was extracted with hot water and ultrasonic extraction, and then precipitated with 80 % ethanol to obtain the crude polysaccharide P80. The extraction rate was 6.81 %. The sugar content of P80 was 46.5 % by phenol sulfuric acid method. The protein of P80 was removed by sevag method, dialysis,freeze-drying and other operations to obtain LGR80, and then the LGR80 was separated by DEAE Focurose 6 FF anion exchange column chromatography to obtain LGR80-1 and LGR80-2. LGR80-1 and LGR80-2 was further purified by Sephadex G-75 gel column to obtain LGRP80-1 and LGRP80-2, respectively. The results of high performance gel permeation chromatography showed that both LGRP80-1 and LGRP80-2 had high purity.