蛋白质印迹技术的研究进展

Research Progress of Protein Imprinting Technology

蛋白质印迹是分子生物学和蛋白质组学中最常用的技术之一。由于蛋白质印迹是一个多步骤实验,任何步骤的错误都可能降低该技术的可靠性和重复性。最近的报告表明,实验中的一些关键步骤,如样品制备方法、所使用一抗的比例和来源,以及所使用的标准化方法,对于可重复的蛋白质印迹结果至关重要。在这篇综述中,总结了蛋白质印迹技术的研究进展,包括蛋白质制备、转模和抗体使用,讨论了最先进的蛋白质印迹技术,并展望了蛋白质印迹技术的发展前景。

Western blotting is one of the most commonly used techniques in molecular biology and proteomics. Since western blotting is a multistep protocol,variations and errors can occur at any step reducing the reliability and reproducibility of this technique. Recent reports suggest that a few key steps, such as the sample preparation method, the amount and source of primary antibody used, as well as the normalization method utilized, are critical for reproducible western blot results.