超高效液相色谱-串联质谱法测定猪肉中14种局部麻醉类药物残留

Determination of 14 local anesthetics in pork by ultra-performance liquid chromatography-tandem mass spectrometry

采用超高效液相色谱-串联质谱技术建立了猪肉中普鲁卡因、利多卡因、苯佐卡因、丁卡因、布比卡因、罗哌卡因、辛可卡因、依替卡因、卡波卡因、布他卡因、美索卡因、奥布卡因、丙美卡因、阿替卡因、丙胺卡因等14种局部麻醉类药物残留分析的方法。经pH 4.0 KH_(2)PO_(4)缓冲液提取,MCX柱净化,BEH C_(18)柱分离,采用0.1%甲酸水和乙腈进行梯度洗脱,正离子模式监测,外标法定量。结果显示,14种局部麻醉类药物在0.2~20.0μg/kg的浓度范围内线性良好,相关系数均大于0.999,检出限为0.05~0.1μg/kg,定量限在0.2~0.5μg/kg之间,药物加标回收率为73.3%~90.7%,RSD小于10%,方法可为局麻药的有效监管、打击注水注药违法行为提供技术支持。

A method based on ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was developed for the simultaneous determination of procaine,lidocaine,benzocaine,tetracaine,bupivacaine,ropivacaine,cinchocaine,eticaine,carbocaine,butacaine,mexocaine,oxybuprocaine,proparacaine,articaine and prilocaine residues in pork.The sample was extracted with a potassium dihydrogen phosphate buffer of pH 4.0,purified by an MCX solid phase extraction column,separated by BEH C_(18)column,eluted by 0.1%formic acid water and acetonitrile,monitored by positive ion mode and quantified by external standard method.The results showed that the 14 local anesthetics had good linearities in the concentration range of 0.2-20.0μg/kg,all the correlation coefficients were greater than 0.999,the limits of detection were 0.05-0.1μg/kg,the limits of quantification were 0.2-0.5μg/kg,the average recoveries were 73.3%-97.7%,and the relative standard deviations were less than 10%.The method could provide technical support for the effective supervision of local anesthetics utilization and cracking down on illegal acts of water injection。