基于Oncomine芯片数据库分析低密度脂蛋白受体相关蛋白4在甲状腺乳头癌中的表达及意义

Analysis of the expression and significance of low-density lipoprotein receptor related protein 4 in papillary thyroid carcinoma based on the Oncomine gene array database

目的研究低密度脂蛋白受体相关蛋白4(LRP4)在甲状腺乳头状癌(PTC)中的表达和意义。方法挖掘Oncomine数据库中关于LRP4的信息,并对目前数据库中资料进行二次分析,探索其在PTC中的表达及意义。结果Oncomine数据库中共收集了400项不同肿瘤中关于LRP4的研究,与正常组织相比,LRP4表达明显增高的有11项,表达明显降低的有7项。共3项研究中的5个数据集包含135例样本涉及LRP4在PTC组织与正常组织中的表达,对这5项研究结果进行Meta分析显示,LRP4在所有差异表达基因中的中位数值排名为11,P=3.06×10^(-8),提示LRP4在PTC中高表达。LRP4在PTC组织中的表达量均明显高于正常组织,差异均有统计学意义(P﹤0.01)。LRP4高表达与低表达患者的生存情况比较,差异无统计学意义(P﹥0.05)。结论通过对Oncomine基因芯片数据库中肿瘤相关基因信息的深入挖掘显示,LRP4在PTC组织中高表达,提示LRP4可能是PTC的致癌基因,为PTC的靶向治疗提供了方向。研究未发现LRP4的高低表达与PTC患者的生存有关。

Objective To study the expression of low-density lipoprotein receptor related protein 4(LRP4)in papil-lary thyroid carcinoma(PTC)and its clinical significance.Method The expression information about LRP4 in the Onco-mine database was mined,and the data in the current database was re-analyzed to explore its expression and clinical sig-nificance in PTC.Result A total of 400 serials on LRP4 in different tumors have been collected in the Oncomine data-base.Among all studies,11 have significantly higher expression of LRP4,and 7 have significantly lower expression com-pared with those in normal tissues.A total of 5 data sets in 3 studies contained 135 samples involving the expression of LRP4 in PTC tissues and normal tissues.Meta-analysis of these five studies showed that the median value of LRP4 among all differentially expressed genes ranked as 11,P=3.06×10^(-8),suggesting that LRP4 was up-regulated in PTC.The expression of LRP4 in PTC tissues was significantly higher than that in normal tissues,and the differences was statistical-ly significant(P<0.01).There was no statistically significant difference in the overall survival of patients with high and low expression of LRP4(P>0.05).Conclusion By in-depth mining of cancer-related gene information in the Oncomine gene express array database,LRP4 is up-regulated in the PTC tissue.This finding suggests that LRP4 may be the onco-gene of PTC,which provides a potential target for PTC treatment.Besides,the study did not find that the expression of LRP4 is related to the survival of PTC patients.