Aux/IAA基因家族鉴定及对大豆茎尖发育的调控作用分析

Identification of Aux/IAA gene family and the regulation to apical bud development in soybean

Aux/IAA基因家族在植物茎尖发育过程发挥重要作用。为了探究大豆Aux/IAA基因家族在大豆茎尖发育过程的调控作用,本文以拟南芥Aux/IAA基因家族蛋白序列为参照鉴定了大豆全基因组Aux/IAA家族基因,包括63个成员;然后以拟南芥、鹰嘴豆和大豆的Aux/IAA家族为研究对象,比对这些基因全长氨基酸序列并构建进化树,结果表明三种作物的Aux/IAA家族成员间亲缘关系差异明显,进化过程中同源重组频率不同;进而利用RNA-seq技术分析东农594(DN)、Charleston(CH)及二者杂交后代的RIL群体的高矮秆池(WH、WS)和F2群体的高矮秆池(JH、JS)的差异表达基因及其功能,共有17个Aux/IAA基因在3组材料中差异表达。CHvsDN组有15个差异表达基因,JHvsJS组有2个,WHvsWS组只有1个;其中,Glyma.10G180100在JHvsJS组和WHvsWS组均差异表达,这些结果为进一步研究大豆Aux/IAA基因家族的功能及调控茎尖发育提供理论依据。

The Aux IAA gene family plays an important role in the development of plant shoots.In order to explore the regulatory role of Aux/IAA gene family in the development process of soybean shoots,the protein sequences of Aux/IAA gene family of Arabidopsis thaliana were used in current study as a reference to identify the Aux/IAA family genes in soybean genome.There were 63 Aux/IAA gene members in soybean genome.Then the full-length amino acid sequences of Aux/IAA family genes identified from Arabidopsis thaliana and chickpea and soybean were compared to construct an evolutionary tree.The results showed that the genetic relationship between Aux/IAA family members was significantly different,and the frequency of homologous recombination during the process of plant evolution was different.The genes were identified which expressed differentially between 594(DN)and Charleston(CH)and between the high pool(WH)and short pool(WS)of their RIL population and the high pool(JH)and short pool(JS)of their F2 population.A total of 17 Aux/IAA genes are differential expression.There were 15 genes expressed differentially in the CHvsDN group,2 in the JHvsJS group,and only 1 in the WHvsWS group.Furthermore,Glyma.10 G180100 was differentially expressed in the JHvsJS group and the WHvsWS group.These results give a deep understanding about the Aux/IAA gene family in soybean and provide a theoretical basis for regulating the development of soybean shoots.