基于ISSR与ITS-RFLP的花生白绢病菌遗传多样性分析

Genetic diversity of Sclerotium rolfsii infecting groundnut based on ISSR and ITS-RFLP

本研究利用简单重复序列区间(Inter Simple Sequence Repeat,ISSR)和ITS-RFLP技术,对采自我国12个省市的39个花生白绢病菌菌株进行遗传多样性分析。结果表明:从筛选出的17条ISSR引物共扩增出269条DNA谱带,其中多态性谱带266条,平均多态性百分率为98.9%,UPGMA聚类分析将这些菌株分为4个类群。限制性内切酶AluⅠ、RsaⅠ、HpaⅡ和MboⅠ作用内部转录间隔区(Internal Transcribed Spacer,ITS)PCR扩增产物产生的谱带,利用ITS-RFLP分析遗传多样性,将39个菌株划分为7个组群。ISSR和ITS-RFLP的结果均表明花生白绢病菌存在较高的遗传变异,但其遗传相似性与地理来源无明显相关。

Inter simple sequence repeat(ISSR)and ITS-RFLP were used to analyze the genetic diversity of 39 Sclerotium rolfsii peanut strains collected from 12 provinces in China.A total of 269 DNA bands were amplified from 17 ISSR primers,266 bands accounting for 98.9%showed polymorphic among them.These strains were grouped into 4 clusters by UPGMA analysis according to ISSR polymorphic bands.Variation in internal transcribed spacers(ITS)was examined following restriction enzyme digests.Restriction fragment length polymorphisms(RFLPs)were observed in ITS fragments digested by AluⅠ,RsaⅠ,HpaⅡand MboⅠ,and divided 39 strains into seven groups.The results of ISSR and ITS-RFLP showed that there was high level of genetic variation among S.rolfsii strains,and genetic groups did not correlate with geographical origin.