LAPTM4B在胰腺癌中的表达与其侵袭、转移及预后的关系

Relationship between the expression of LAPTM4B and its invasion,metastasis and prog⁃nosis in patients with pancreatic cancer

目的探讨溶酶体相关4次跨膜蛋白B(LAPTM4B)在胰腺癌组织中的表达水平,并分析LAPTM4B表达水平对癌细胞侵袭及转移的影响。方法选取2015年1月至2019年1月本院收治的96例胰腺癌患者,采用免疫组化染色法检测LAPTM4B在胰腺癌组织及癌旁正常组织中的表达情况,分期与病理特征的关系。Kaplan⁃Meier生存分析LAPTM4B表达对胰腺癌患者预后生存的影响,使用tarone法进行检验,Logistic回归分析影响患者预后的相关因素。结果观察组LAPTM4B阳性表达率为76.04%,显著高于对照组54.17%,差异具有统计学意义(P<0.05)。LAPTM4B表达与TNM分期、淋巴结转移、分化程度有关,TNM分期为Ⅲ~Ⅵ期、有淋巴结转移、分化程度为中⁃低分化的胰腺癌患者LAPTM4B阳性表达率更高(P<0.05)。96例胰腺癌患者1年生存率为20.83%(20/96),LAPTM4B阳性表达的胰腺癌患者死亡率更高(P<0.05)。LAPTM4B表达阴性及表达阳性半数生存时间分别为(8.30±0.73)个月、(6.82±0.51)个月。生存分析显示LAPTM4B表达阳性的患者生存期明显短于LAPTM4B表达阴性的患者,Keplan⁃meier生存曲线比较差异具有统计学意义(P<0.05)。TNM分期为Ⅲ~Ⅵ期、有淋巴结转移、分化程度为中⁃低分化、LAPTM4B表达阳性为影响胰腺癌患者预后生存的独立危险因素(P<0.05)。结论LAPTM4B在胰腺癌的侵袭及转移中发挥重要作用,与胰腺癌患者预后密切相关,可作为评估胰腺癌患者预后的重要检测标志物。

Objective To explore the expression of lysosome⁃associated transmembrane protein 4B(LAPTM4B)in pancreatic cancer tissues,and analyze the effect of LAPTM4B expression on the invasion and metastasis of cancer cells.Methods The 96 patients with pancreatic cancer admitted to this hospital from January 2015 to January 2019 were selected.Immunohistochemical staining was used to detect the expression of LAPTM4B in pancreatic cancer tissues and adjacent normal tissues and to detect the relationship between staging and pathological features.Kaplan⁃Meier survival curve was used to analyze the effect of LAPTM4B ex⁃pression on the prognosis survival of patients with pancreatic cancer.The tarone method was used for testing,and the logistic regression analysis was used to analyze the relevant factors affecting the prognosis of patients.Results The positive expression rate of LAPTM4B in the observation group was 76.04%,which was signifi⁃cantly higher than 54.17%in the control group.The difference was statistically significant(P<0.05).The expression of LAPTM4B was related to TNM staging,lymph node metastasis,and degree of differentiation.The positive expression rate of LAPTM4B in pancreatic cancer patients with TNM stagesⅢ⁃Ⅵ,lymph node metas⁃tasis,and moderate⁃to⁃low differentiation was higher(P<0.05).The 1⁃year survival rate of 96 patients with pancreatic cancer was 20.83%(47/96),and the mortality of pancreatic cancer patients with positive expression of LAPTM4B was higher(P<0.05).The half survival time of LAPTM4B negative and positive expression was(8.30±0.73)months and(6.82±0.51)months,respectively.Survival analysis showed that the survival time of patients with positive LAPTM4B expression was significantly shorter than that of patients with negative LAPTM4B expression,and the difference in Keplan⁃meier survival curve was statistically significant(P<0.05).TNM staging ofⅢtoⅥ,lymph node metastasis,moderate to low differentiation,and positive LAPTM4B expression were independent risk factors affecting the prognosis survival of patients with pancreatic cancer(P<0.05).Conclusion LAPTM4B plays an important role in the invasion and metastasis of pancreatic cancer,is closely related to the prognosis of pancreatic cancer patients,and can be used as an important detec⁃tion marker for evaluating the prognosis of pancreatic cancer patients.