ERCC1、PAK、HDAC1蛋白在NSCLC的表达及临床意义

Expression and clinical significance of ERCC1,PAK and HDAC1 protein in patients with NSCLC

目的探讨免疫组化检测核苷酸切除修复交叉互补组1(ERCC1)、P21活化激酶(PAK)及组蛋白去乙酰化酶1(HDAC1)蛋白在非小细胞肺癌(NSCLC)患者中的表达及临床意义。方法纳入2015年1月至2017年6月于本院接受手术治疗的62例NSCLC患者,将术中切取的癌组织作为癌组织组,将癌旁正常组织作为癌旁正常组织组,检测ERCC1、PAK及HDAC1蛋白的表达水平。对所有患者的临床病理特征、ERCC1、PAK及HDAC1蛋白表达情况及预后情况进行记录。采用ROC曲线研究ERCC1、PAK及HDAC1蛋白对NSCLC患者预后生存的影响。结果癌组织组ERCC1、PAK及HDAC1蛋白阳性率表达显著高于癌旁正常组织组,差异有统计学意义(P<0.05)。ERCC1、PAK及HDAC1蛋白高表达者临床分期、组织分化程度及淋巴结转移明显高于低表达者,差异有统计学意义(P<0.05)。62例NSCLC患者3年生存率为19.35%(12/62),ERCC1、PAK及HDAC1高表达者死亡率高于低表达者,差异有统计学意义(P<0.05)。ERCC1、PAK及HDAC1蛋白及三者联合曲线下面积分别为0.786、0.770、0.748、0.973,以联合检测最大(P<0.05)。结论ERCC1、PAK及HDAC1蛋白在NSCLC癌组织中呈异常表达状态,临床工作者可通过监测上述指标判断NSCLC患者疾病进展情况,为评估患者预后发展提供参考。

Objective To explore the expression and clinical significance of immunohistochemi⁃cal detection of nucleotide excision repair cross⁃complementation group 1(ERCC1),P21 activated kinase(PAK)and histone deacetylase 1(HDAC1)protein in patients with non⁃small cell lung cancer(NSCLC).Methods 62 patients with NSCLC who underwent surgical treatment in this hospital from January 2015 to June 2017 were included.The cancer tissue removed during the operation was used as the cancer tissue group,and the adjacent normal tissues were used as the adjacent normal tissue group.The expression levels of ER⁃CC1,PAK and HDAC1 protein were detected.The clinicopathological characteristics,ERCC1,PAK and HDAC1 protein expression and prognosis of all patients were recorded.The ROC curve was used to study the effect of ERCC1,PAK and HDAC1 protein on the prognostic survival of patients with NSCLC.Results The positive rates of ERCC1,PAK and HDAC1 protein expression in the cancer tissue group were significantly higher than those in the adjacent normal tissue group,and the difference was statistically significant(P<0.05).The clinical stage,degree of tissue differentiation and lymph node metastasis of patients with high expression of ERCC1,PAK and HDAC1 protein were significantly higher than those of patients with low expression,and the difference was statistically significant(P<0.05).The 3⁃year survival rate of 62 NSCLC patients was 19.35%(12/62),and those with high expression of ERCC1,PAK and HDAC1 had higher mortality,and the difference was statistically significant(P<0.05).The areas under the curves of ERCC1,PAK and HDAC1 pro⁃tein and their combined detection were 0.786,0.770,0.748,0.973,respectively,and the combined detection was the largest(P<0.05).Conclusion ERCC1,PAK,and HDAC1 proteins are abnormally expressed in NSCLC cancer tissues.Clinicians can monitor these three indicators to judge the disease progression of NSCLC patients,and provide references for assessing the prognosis and development of patients.