摘要
由于缺乏明确的二倍体供体信息,燕麦属植物的起源和系统进化关系一直存在争议。利用荧光原位杂交(fluorescence in situ hybridization,FISH)方法,检测45S rDNA和5S rDNA在燕麦属不同倍性植物染色体上的位点信息;并依据已公开的45S rDNA ITS区全长DNA序列构建分子进化树。探讨燕麦属植物在不同基因组中45S rDNA的位点变化、进化规律以及分化机制,为探究燕麦属物种的起源与演化提供参考。
Due to the incomplete information on the diploid donor, the origin and phylogenetic relationship of oats have been controversial. The fluorescence in situ hybridization(FISH) method was used to detect the location information of 45 S rDNA and 5 S rDNA on the chromosomes of different ploidy plants of Avena. The molecular evolution tree was constructed based on the published full-length DNA sequence of the 45 S rDNA ITS region. This paper discussed the locus changes, evolution laws, and differentiation mechanisms of 45 S rDNA in different genomes of oats plants and provided references for exploring the origin and evolution of oat species.
作者
王炳策
刘晓娟
程斌
任明见
徐如宏
张素勤
张立异
何方
Wang Bingce;Liu Xiaojuan;Cheng Bin;Ren Mingjian;Xu Ruhong;Zhang Suqin;Zhang Liyi;He Fang(College of Agriculture,Guizhou University/Guizhou Subcenter of National Wheat Improvement Center,Guiyang 550025,Guizhou,China;Institute of Upland Crops,Guizhou Academy of Agriculture Sciences,Guiyang 550006,Guizhou,China)
出处
《作物杂志》
北大核心
2021年第4期10-17,共8页
Crops
基金
国家自然科学基金地区项目“利用Seq-BSA技术准确定位小麦新抗源贵协3号的抗条锈病基因”(31660393)
贵州省基金重点项目“小麦抗源贵协3号抗条锈病基因YrGX3的精细定位”(黔科合基础[2019]450号)
贵州省农业科学院青年科技基金“小麦贵协3号抗条锈病基因的细胞学定位分析”(黔农科院青年基金[2018]02号)。
