马铃薯种质资源晚疫病抗性评价及分子标记辅助筛选

Evaluation of Germplasms for Resistance to Potato Late Blight and Molecular Markers Assisted Screening

由致病疫霉(Phytophthora infestans)引起的晚疫病是马铃薯生产上最具危害性的病害,种植抗病品种是防治该病害最有效的方法。分子标记辅助选择可以大大缩短育种年限,提高育种效率。对395008.45×克新27号的60份杂交后代进行了晚疫病抗性评价,结果有39份表现抗病,21份表现感病。利用晚疫病抗性基因R8的分子标记对60份杂交后代进行了检测,其中有43份含有R8基因;对36份种质资源进行检测,其中有28份含有R8基因。本研究鉴定的抗性资源可为马铃薯抗病育种提供优质的亲本材料。

Potato late blight caused by Photophthora infestans has always been a devastating disease in potato production. Use of resistant varieties is the most fundamental and effective way to control the disease. Molecular marker assisted selection can greatly shorten the breeding period, significantly improve the breeding efficiency. A total of 60 offspring of hybrid combinations of 395008.45×Kexin No.27 were evaluated for resistance to Phytophthora infestans. The results showed that 39 materials were resistantance varieties and 21 materials were susceptible varieties. The molecular marker related to the resistance gene R8 were used to detect 60 offspring and 36 potato germplasm resources, and 43 varieties(lines) and 28 varieties(lines) had resistance gene R8, respectively. The potato resistance germplasm resources tested could provide high-quality parental materials for disease resistance breeding.