基于UPLC-Q.Exactive-MS技术分析高羊茅在萘胁迫下的代谢组学影响

Analysis of the Metabonomics Effect of Tall Fescue Under Naphthalene Stress Based on UPLC-Q·Exactive-MS Technology

萘作为典型的稠环芳烃,其在环境中的行为引起了人们的广泛关注。高羊茅适应性强且抗逆性突出,已被证明具有降解多环芳烃的能力。以高羊茅为研究对象,在水培环境下以100 mg/L萘对高羊茅进行胁迫处理,分别在第12 h,3 d和9 d时取样,通过UPLC-Q·Exactive-MS技术分析,结合Mzmine软件、MetaboAnalyst 5.0网站以及KEGG数据库,探究高羊茅在萘胁迫下的代谢组学影响,推断出萘的两种代谢产物分别为异喹啉(Isoquinoline)、苯并呋喃(Benzofuran)。

As a typical polycyclic aromatic hydrocarbon,the behavior in the environment has attracted widespread attention.Tall fescue has strong adaptability and outstanding stress resistance,and has been proven to have the ability to degrade polycyclic aromatic hydrocarbons.Taking tall fescue as the research object,stresses tall was stressed with 100 mg/L naphthalene in a hydroponic environment,and samples were taken at 12 h,3 d and 9 d respectively.Through UPLC-Q·Exactive-MS technical analysis,combined with Mzmine software,MetaboAnalyst 5.0 website and KEGG database,the metabonomic effects of tall fescue under naphthalene stress were explored,and the two metabolites of naphthalene were inferred to be isoquinoline and benzofuran.