甲苯噻嗪改良小鼠癫痫持续状态模型的研究

Improvement of status epilepticus by xylazine in mice

目的探索一种高成功率和高生存率的小鼠癫痫持续状态改良模型。方法在传统匹鲁卡品诱导模型的基础之上,加用肌肉松驰药甲苯噻嗪,观察甲苯噻嗪不同剂量、不同给药时间对小鼠癫痫发作等级、造模成功率、死亡率与生存率的影响,并采用实时荧光PCR检测部分小鼠海马c-Fos mRNA的表达水平。结果甲苯噻嗪4 mg·kg^(-1)预处理组中,小鼠发作等级、海马c-Fos mRNA水平与传统模型组相比差异无统计意义,而生存率和成功率明显高于传统模型组;甲苯噻嗪8 mg·kg^(-1)预处理组模型成功率明显低于传统模型组;而甲苯噻嗪后处理组动物生存率与成功率与传统模型组相比差异无统计学意义。结论甲苯噻嗪4 mg·kg^(-1)预处理能够显著提高模型的成功率和动物生存率,是一种高效、易于重复的构建小鼠癫痫持续发作状态模型的方法。

Objective To explore a new model of status epilepticus in mice with a high success rate and high survival rate.Methods Based on the traditional induction model with pilocarpine in combination of a muscle relaxant xylazine,the effects of different doses and dosing times on the seizure grade,success rate,mortality and survival rate of mice with status epilepticus were recorded.The expression of hippocampal c-Fos mRNA was examined using real-time fluorescence PCR in some mice.Results No statistical difference was found in seizure grade and the level of hippocampal c-Fos mRNA between the xylazine 4 mg.kg^(-1) pretreatment group and the traditional model group.The success rate was significantly lower in the xylazine 8 mg.kg^(-1) preteatment group than those in the traditional model group.There was no difference in the survival rate and success rate between the xylazine pretreatment group and the traditional model group.Conclusions Pretreatment with 4 mg·kg^(-1)xylazine significantly improves the success rate and animal survival rate,which is an efficient and repeatable method to establish a status epilepticus model of mice.