丙烯酰胺对小鼠血液毒性的研究

Study of the toxic effects of acrylamide in mice

目的为探明丙烯酰胺对小鼠血液的毒性效应。方法采用丙烯酰胺溶液连续灌胃小鼠16天,记录小鼠体重、器官系数变化并对小鼠血液成分进行检测。结果当染毒剂量为120、150 mg/kg时,小鼠体重增重率、肝系数、WBC、LYM、RBC、MCHC、PLT、P-LCR显著降低(P<0.05),GRAN显著增加(P<0.05);当丙烯酰胺浓度为90、120、150 mg/kg时,小鼠肾系数、HGB下降显著(P<0.05);当丙烯酰胺浓度为150 mg/kg时,MCV、PCT显著下降(P<0.05);当丙烯酰胺浓度≥60 mg/kg时,AST、ALT、AKP、CREA增加显著(P<0.05),同时当丙烯酰胺浓度为60 mg/kg时,AST活性最高;当染毒剂量≥90 mg/kg时,UA、BUN含量增加显著(P<0.01),当染毒剂量达到120 mg/kg时,BUN含量最大。结论丙烯酰胺对小鼠血液有一定得毒性效应。中高浓度的丙烯酰胺(≥90 mg/kg)会对小鼠红细胞系统、白细胞系统、肝、肾等器官产生一定的损伤效应;高浓度的丙烯酰胺(≥120 mg/kg)会对血小板系统造成明显损伤。

To evaluate the toxicity of circulating acrylamide in mice.Methods Mice were gavaged daily with acrylamide solution for 16 days.Their body and organ masses were recorded and relevant blood parameters were measured.Results Doses of 120 or 150 mg/kg acrylamide daily caused significant reductions in body mass gain,relative liver mass,WBC,LYM,RBC,MCHC,PLT,and P-LCR of the mice(P<0.05),whereas GRAN increased significantly(P<0.05).Doses of acrylamide of 90,120,and 150 mg/kg caused significant reductions in the relative kidney mass and HGB(P<0.05).A dose of 150 mg/kg significantly reduced MCV and PCT(P<0.05).Doses≥60 mg/kg caused significant increases in AST,ALT,AKP,and CREA(P<0.05),and when the concentration was 60 mg/kg,the AST activity was highest.Doses≥90 mg/kg caused significant increases in UA and BUN(P<0.01),and when the dose was 120 mg/kg,the BUN was highest.Conclusions Acrylamide has toxic effects in mice.Medium and high doses of acrylamide(≥90 mg/kg)have deleterious effects on red blood cells,white blood cells,the liver,kidney,and other organs in mice.High concentrations of acrylamide(≥120 mg/kg)also have significant deleterious effects on platelets.