烟草叶蛋白提取技术研究进展

Research Progress on Extraction Technology of Tobacco Leaf Proteins

烟草除了可以作为卷烟原料外,其叶蛋白也是世界上最丰富的蛋白质资源之一。烟叶中的蛋白质分为可溶性蛋白质和不溶性蛋白质,其中约一半的可溶性蛋白是FI蛋白(Fraction I protein),另一半是FII蛋白(Fraction II protein)。随着科学的发展,许多新技术已应用于蛋白质分离,如双水相萃取法、超滤法、层析法、电泳法等。本文根据国内外相关研究资料,对目前分离烟草叶蛋白常用的几种提取技术及进展进行了综述和讨论,并展望了其应用前景。

Tobacco is the raw material of cigarettes in general,but its leaf is one of the most abundant protein resources in the world.The proteins in tobacco leaves can be divided into water-soluble and water-insoluble proteins.Half of the soluble proteins are FI proteins(Fraction I proteins),and the other half are FII proteins(Fraction II proteins).With the development of technology,many new technologies have been applied to protein separation,such as aqueous two-phase extraction,ultrafiltration,chromatography,and electrophoresis.According to the relevant research literatures at home and abroad,several commonly used extraction techniques and the latest progress for separating tobacco leaf proteins were reviewed and discussed in this article,and their application prospects were described.