高效液相色谱法测定槐仙颗粒中黄芩苷的含量

Determination of Baicalin in Huaixian Granules by HPLC

目的:建立槐仙颗粒中黄芩苷的含量测定方法,为丰城市中医院院内制剂槐仙颗粒备案提供技术依据。方法:采用高效液相色谱法,十八烷基硅烷键合硅胶为填充剂的拓谱C18柱(4.6 mm×250 mm,5μm),甲醇-水-磷酸(47∶53∶0.2)为流动相,流速1 mL/min,检测波长为280 nm,柱温35℃。结果:黄芩苷在0.2892μg~1.1568μg范围内具有良好线性关系,线性方程Y=4000000X-18033,R2=1。精密度RSD为0.63%,稳定性试验RSD为0.26%,重复性试验RSD为0.65%,平均回收率为99.09%。结论:对槐仙颗粒中的黄芩苷进行定量,所建方法简便,灵敏,重现性好,可有效控制槐仙颗粒制剂质量。

Objective:To establish a method for the determination of baicalin in Huaixian granules,and to provide a technical basis for the preparation record of Huaixian granule in Fengcheng Hospital of Traditional Chinese Medicine.Methods:The C18 column(4.6 mm×250 mm,5μm)was expanded with octadecylsilane bonded silica gel as the filling agent.The mobile phase was methanol-water-phosphoric acid(47∶53∶0.2)at the flow rate of 1 mL/min.The detection wavelength was set at 280 nm and the column temperature was 35℃.Results:Baicalin had a good linear relationship in the range of 0.2892μg-1.1568μg,the linear equation Y=4000000X-18033,R2=1.RSD of precision,stability and reproducibility tests were 0.63%,0.26%and 0.65%,respectively.The average recovery was 99.09%.Conclusion:The established method for quantitative determination of baicalin in Huaixian granules is simple,sensitive and reproducible,and can effectively control the quality of Huaixian granules.