8个玉米DOF家族基因对非生物胁迫的响应模式分析

Expression pattern analysis of 8 ZmDOFs under abiotic stress in maize

以8个玉米DOF基因为研究对象,研究这8个基因在玉米生长发育中的器官表达模式及应答4种逆境胁迫(200 mmol·L^(-1) NaCl,20%PEG,铵态氮胁迫和硝态氮胁迫)的响应表达模式。结果表明,7个基因具有明显的组织表达特异性,5个基因主要在玉米1种器官中表达,2个基因主要在2种器官中表达,而ZmDOF37呈组成性表达。这些数据表明,这8个基因参与玉米器官的发育进程。在200 mmol·L^(-1) NaCl胁迫处理和20%PEG6000胁迫处理1 h后,分别有8个基因和4个基因表现上调,表明这些基因是NaCl和PEG胁迫响应信号途径中的早期响应基因。在20%PEG6000胁迫处理24 h后,有4个基因被明显的诱导(ZmDOF29,ZmDOF32,ZmDOF37和ZmDOF45),表明这4个基因属于晚期响应基因。在铵态氮和硝态氮胁迫处理1 h后,分别有7个基因被明显诱导,属于早期响应基因,表明这7个基因可能参与玉米应答氮胁迫的早期响应机制。

In this study,8 ZmDOFs,belonging to the same gene subgroup,were used for studying the tissue-specific expression and expression patterns under four abiotic treatments,including 200 mmol·L^(-1) NaCl,20%PEG treatment,ammonium nitrogen deficient and nitrate nitrogen deficient.The results showed that 7 ZmDOFs had tissue specific expression,with 5 ZmDOFs in one tissue,2 ZmDOFs on two tissues.ZmDOF37 had constitutive expression pattern.After 1 h under 200 mmol·L^(-1) NaCl or 20%PEG treatment,the expression of 8 and 4 ZmDOFs obviously upregulated,respectively,indicating these ZmDOFs are early response genes.After 24 h treatment under 20%PEG treatment,4 ZmDOFs(ZmDOF29,ZmDOF32,ZmDOF37 and ZmDOF45)were apparently induced,indicating these genes are delayed-response genes.After 1 h under ammonium and nitrogen deficient treatment,7 ZmDOFs were obviously upregulated,indicating these genes are early-response genes and are likely to have been involved in the early response mechanism to nitrogen deficient conditions.The results in this study provided a basis for further revealing the functions of ZmDOFs in abiotic stress response,and could facilitate the screening of ZmDOFs which are stress-resistant in maize.