巴马香猪回肠、结肠消化特征研究

Digestive Characteristics of Ileum and Colon in Bama Xiang Pigs

本试验旨在研究巴马香猪回肠、结肠的消化特征。采集了9头成年巴马香猪母猪回肠和结肠内容物,通过超高压液相色谱-串联质谱技术进行质谱检测。使用Compound Discover 3.1软件进行代谢物检测,SMICA-P软件和SPSS 19.0软件进行极显著差异代谢物筛选,MetaboAnalyst 5.0、HMDB和KEGG网站进行代谢物性质和代谢途径分析。结果表明:本研究正、负离子模式分别获得了26119和16265个质谱峰,分别匹配了6403和1912种代谢物;无监督主成分分析中模型对X变量的解释率超过0.6,且9个质量控制样本均集中分布在中心点;监督正交偏最小二乘判别分析中模型对Y变量的解释率和模型预测能力均超过为0.93;200次迭代验证的模型回归直线截距均小于0.05;共检测到42种极显著差异代谢物,其中10个极显著差异代谢物与胆汁酸类物质有关,6个差异代谢物为氨基酸;14种差异代谢物参与了19条代谢途径;胆汁酸类物质在回肠内容物中含量比结肠高90~20574倍,氨基酸类物质含量在回肠内容物中比结肠高5~21倍,十二烷二酸和3-甲基二氧吲哚含量在结肠内容物中比回肠高约1000倍。结果提示,高胆汁酸和高氨基酸吸收效率是回肠的消化特征;高微生物代谢产物是结肠消化特征。

This experiment was conducted to investigate the digestive characteristics of ileum and colon in Bama Xiang pigs.The ileum and colon contents of nine adult Bama Xiang sows were collected and detected by ultra-high performance liquid chromatography-tandem mass spectrometry.Compund Disover 3.1 software was used for metabolite detection,and the significant difference metabolites were screen by SIMCA-P and SPSS 19.0 software.MetaboAnalyst 5.0,HMDB and KEGG websites were used to analyze the metabolite properties and metabolic pathways.The results showed that a total of 26119 and 16265 peaks were obtained in positive and negative ion modes,and 6403 and 1912 metabolites were matched,respectively.The R2X model interpretation of PCA analysis was more than 0.6 and all the nine QC samples were concentrated in the center.The R2Y and Q2 of OPLS-DA analysis were more than 0.93 and the intercepts of Q2 verified by 200 iterations were all less than 0.05.A total of 42 metabolites with extremely significant difference were detected,of which 10 were related to bile acids and 6 were amino acids,and 14 different metabolites were involved in 19 metabolic pathways.The content of bile acids in ileum contents was 90 to 20574 times higher than that in colon,and the content of amino acids in ileum contents was 5 to 21 times higher than that in colon.Instead,both dodecanedioic acid and 3-methyldioxyindole were about 1000 times higher in colonic contents than in ileal contents.The results indicate that the high absorption efficiency of bile acids and amino acids are the digestive characteristics of ileum,and high microbial metabolites is the characteristic of colon digestion.