河野脂螨发育各阶段外部形态扫描电镜观察

Scanning electron microscopic observation of the external morphology of Lardoglyphus konoi(Acariformes:Acaridida:Lardoglyphidae)at different developmental stages

【目的】了解河野脂螨Lardoglyphus konoi各发育阶段外部形态和超微结构特征。【方法】利用室内培养获得的不同发育阶段的河野脂螨个体样本,在扫描电镜下观察各发育阶段(卵、幼螨、若螨和成螨)的外部形态和超微结构。【结果】河野脂螨卵呈椭圆形;幼螨足3对,未见生殖器官生长痕迹,生殖毛和肛毛缺如;若螨足4对,生殖器官发育不完全,有生殖毛和肛毛,但发育亦不完全。休眠体前端尖细,后端钝圆,呈梨形状。在末体区有吸盘板,其上有13个吸盘,呈2-2-4-5分布。成螨螯肢细长,剪刀状,其上的定趾和动趾上有几个小齿;顶外毛长度约为顶内毛的50%,与顶内毛处同一水平线上。雄成螨第3对足的跗节末端为2个粗钝的齿所替代;腹面有1个生殖区和带有1对肛门吸盘的肛区。雌成螨所有的爪均分叉;腹面生殖孔为1个纵向裂缝;肛门不伸达后缘。【结论】通过电镜扫描对河野脂螨不同发育阶段外部形态和超微结构的观察,更加详细地了解了该螨的形态结构,从而为脂螨科螨类的分类和生物学研究提供了理论依据。

【Aim】To observe the external morphology and ultrastructure characteristics of Lardoglyphus konoi at different developmental stages.【Methods】Based on the individual samples of different developmental stages of L.konoi cultured in the laboratory,the external morphology and ultrastructure of various developmental stages of L.konoi,including egg,larva,nymph and adult,were observed under scanning electron microscope(SEM).【Results】The egg of L.konoi is oval.The larva has three pairs of legs,but has no genital organs,genital setae and anal setae.The nymph has four pairs of legs,and the genital area,genital setae and anal setae still look under-developed.The hypopus has a tapering front end and a round and pear-shaped rear end.Sucker plate appears in the end idiosoma,and there are 13 suckers on the sucker plate with 2-2-4-5 distribution.The chelicera of adult mite is slender,scissor-like,and there are several small teeth on its fixed digit and movable digit.The external vertical seta(ve)is about half the length of the internal vertical seta(vi),and situated at the same horizon as the internal vertical seta.Tarsus end of the 3rd leg in the male adult is replaced by two thick,blunt teeth.At the ventral idiosoma,there is a genital region and an anal region with a pair of anal suckers.All legs in the female adult have forked claws.The ventral gonopore is a longitudinal crack.The anus does not reach the posterior margin of idiosoma.【Conclusion】The external morphology and ultrastructure of L.konoi at different developmental stages are observed by SEM,and the morphological characteristics of the mite are understood in more details,providing a theoretical basis for further study of its taxonomy and biology.