口腔扁平苔藓患者外周血中ASH1L及其相关调控因子表达与免疫功能相关性

Correlation between expression of ASH1L and its related regulatory factors in peripheral blood of patients with oral lichen planus and immune function

目的分析口腔扁平苔藓(OLP)患者外周血中ASH1L及其相关调控因子表达与免疫功能的相关性,探讨其在OLP发病机制中的作用及意义。方法收集2018年8月至2019年8月贵州医科大学附属口腔医院40例OLP患者(OLP组)和20名同期体检健康者(对照组)的外周血。采用流式细胞术及散射比浊法检测两组细胞免疫及体液免疫相关指标水平,实时荧光定量PCR检测两组外周血单个核细胞中ASH1L、泛素编辑酶A20、白细胞介素-6(IL-6)mRNA相对表达量及其与OLP免疫指标的相关性。结果OLP组中CD3^(+)、CD3^(+)CD4^(+)、CD3^(+)CD8^(+)、总T^(+)B^(+)NK细胞、IgM水平均低于对照组,CD19^(+)、CD4^(+)/CD8^(+)、补体C3及C4水平均高于对照组,差异有统计学意义(P<0.05)。OLP组ASH1L及A20 mRNA相对表达量低于对照组,IL-6 mRNA高于对照组,差异有统计学意义(P<0.05)。OLP组ASH1L mRNA相对表达量与IgG水平呈正相关(r>0,P<0.05)。A20 mRNA相对表达量与CD8^(+)、IgA水平呈正相关(r>0,P<0.05),与CD4^(+)/CD8^(+)呈负相关(r<0,P<0.05)。结论ASH1L及其相关调控因子在OLP患者外周血中表达异常且与免疫指标水平相关,在OLP免疫相关发病机制中发挥了一定作用。

Objective To analyze the correlation between the expression of ASH1L and its related regulatory factors in the peripheral blood of patients with oral lichen planus(OLP)and the immune function,and to explore its role and significance in the pathogenesis of OLP.Methods The peripheral blood of 40 OLP patients(OLP group)and 20 healthy volunteers(control group)were collected from the Stomatological Hospital Affiliated to Guizhou Medical University from August 2018 to August 2019.Flow cytometry and scattering turbidimetry were used to detect the levels of cellular immunity and humoral immunity.The relative expression levels of ASH1L,ubiquitin editing enzyme A20,and interleukin-6(IL-6)mRNA in peripheral blood mononuclear cells of the two groups and their correlation with OLP immune indexes were detected by quantitative real-time PCR.Results The levels of CD3^(+),CD3^(+)CD4^(+),CD3^(+)CD8^(+),total T^(+)B^(+)NK cells,and IgM in OLP group were lower than those in control group,and CD19^(+),CD4^(+)/CD8^(+),complement C3,and C4 levels were lower than those in control group,with statistical significance(P<0.05).The relative expression levels of ASH1L and A20 mRNA in OLP group were lower than those in control group,and IL-6 mRNA in OLP group was higher than that in control group,with statistical significance(P<0.05).The relative expression of ASH1L mRNA in OLP group was positively correlated with the level of IgG(r>0,P<0.05).The relative expression of A20 mRNA was positively correlated with the levels of CD8^(+)and IgA(r>0,P<0.05),and negatively correlated with the CD4^(+)/CD8^(+)(r<0,P<0.05).Conclusion The abnormal expression of ASH1L and its related regulatory factors in the peripheral blood of OLP patients is correlated with the level of immune indicators,and plays a certain role in the immune-related pathogenesis of OLP.