长链非编码RNA FAL1对胃癌细胞增殖和凋亡能力的影响及机制研究

Study of effects and mechanism of long non-coding RNA FAL1 on the proliferation and apoptosis of gastric cancer cells

目的探讨长链非编码RNA FAL1对胃癌增殖和凋亡的影响及机制。方法正常胃黏膜细胞GES-1和胃癌细胞SGC-7901,BGC-823取自辽宁省肿瘤医院中心实验室,RT-PCR检测细胞中FAL1、STAT3的表达;Western-blot检测STAT3蛋白的表达;在胃癌细胞中抑制FAL1、过表达STAT3,应用CCK-8与Annexin V-FITC PI双染评估细胞增殖和凋亡能力。结果FAL1在人胃癌细胞株SGC-7901和BGC-823中的表达水平高于胃黏膜细胞GES-1中表达,差异有统计学意义(P<0.05)。STAT3在SGC-7901和BGC-823中的表达水平高于GES-1,差异有统计学意义(P<0.05)。转染后胃癌细胞中FAL1表达水平降低(P<0.05)。胃癌细胞中STAT3的表达水平同样下降(P<0.05)。将si-FAL1#2序列转染SGC-7901和BGC-823细胞后,细胞增殖减缓,在此基础上过表达STAT3,被抑制的细胞的增殖能力部分恢复(P<0.05);细胞早期凋亡明显增加,过表达STAT3后,凋亡细胞降低(P<0.05)。结论FAL1通过上调STAT3的表达促进胃癌细胞增殖并抑制凋亡。

Objective To explore the effect and mechanism of long non-coding RNA FAL1 on the proliferation and apoptosis of gastric cancer.Methods Normal gastric mucosal cells GES-1 and gastric cancer cells SGC-7901,BGC-823 were taken from the Central Laboratory of Liaoning Cancer Hospital&Institute,and RT-PCR was used to detect the expression of FAL1 and STAT3 in the cells;Western-blot was detected the expression of STAT3 protein;inhibition of FAL1 and overexpression of STAT3 in gastric cancer cells,double staining with CCK-8 and Annexin V-FITC PI were evaluated cell proliferation and apoptosis.Results The expression levels of FAL1 in human gastric cancer cell lines SGC-7901 and BGC-823 were higher than that in gastric mucosal cells GES-1,and the differences were statistically significant(P<0.05).The expression levels of STAT3 in SGC-7901 and BGC-823 were higher than that in GES-1,and the differences were statistically significant(P<0.05).The expression level of FAL1 in gastric cancer cells was decreased after transfection(P<0.05).The expression level of STAT3 in gastric cancer cells was also decreased(P<0.05).After the si-FAL1#2 sequence was transfected into SGC-7901 and BGC-823 cells,cell proliferation was slowed down.On this basis,over-expression of STAT3,the proliferation ability of the inhibited cells was partially restored(P<0.05);early cell apoptosis increased significantly,after overexpression of STAT3,apoptotic cells were decreased(P<0.05).Conclusion FAL1 promotes the proliferation of gastric cancer cells and inhibits apoptosis by up-regulating the expression of STAT3.