溃疡性结肠炎组织中TET2的表达水平与患者预后的关系

Relationship between expression of TET2 in ulcerative colitis and prognosis

目的探究溃疡性结肠炎(UC)组织中DNA甲基胞嘧啶双加氧酶10-11易位2(TET2)的表达水平与患者预后的关系。方法选择2018年10月至2019年10月在遂宁市中心医院就诊的102例UC患者作为研究对象。采用蛋白质印迹法检测UC组织中TET2的表达水平,并分析其与UC患者预后的关系。结果102例UC患者中有41例(40.20%)预后不良。根据预后情况将患者分为预后良好组(n=61)和预后不良组(n=41)。预后良好组TET2的表达水平低于预后不良组,差异有统计学意义(P<0.05)。多因素logistic回归分析结果显示,改良Mayo评分、IL-1β、TET2和初次治疗应用糖皮质激素均是UC患者预后的独立危险因素(P均<0.05)。模型B(由改良Mayo评分、IL-1β、TET2和初次治疗应用糖皮质激素组成)评估UC患者预后的效能高于模型A(由改良Mayo评分、IL-1β和初次治疗应用糖皮质激素组成),差异有统计学意义(P<0.05)。当阈值概率>10%时,模型B评估UC患者预后的净收益高于模型A。结论UC组织中TET2的表达水平与患者预后有关。基于TET2构建的模型对于评估UC患者的预后情况具有一定价值。

Objective This paper aims to explore the relationship between DNA methylcytosine dioxygenase 10-11 translocation 2(TET2)in ulcerative colitis(UC)tissue and the prognosis of patients.Methods A total of 102 patients with UC who were treated in Suining Central Hospital from October 2018 to October 2019 were selected as study subjects.The level of TET2 in UC tissues was detected by Western blotting,and its relationship with prognosis of UC patients was analyzed.Results Of the 102 UC patients,41(40.20%)have poor prognosis.The subjects were divided into two groups according to UC prognosis:the good prognosis group(n=61)and the poor prognosis group(n=41).The level of TET2 in the good prognosis group is lower than that of the poor prognosis group,with a statistically significant difference(P<0.05).The logistic regression analysis shows that the modified Mayo score,IL-1β,TET2,and initial treatment application of glucocorticoids are independent risk factors for prognosis of UC(P<0.05).Model B(consisting of modified Mayo score,IL-1β,TET2,and initial treatment application of glucocorticoids)is more effective in evaluating the prognosis of UC than Model A(consisting of modified Mayo score,IL-1β,and initial treatment application of glucocorticoids),with a statistically significant difference(P<0.05).When the threshold probability is greater than 10%,the net benefit of Model B in evaluating the prognosis of UC is higher than that of Model A.Conclusions The level of TET2 in UC tissue is related to prognosis.The model based on TET2 has certain values in evaluating the prognosis of UC patients.