鸡滑液囊支原体河南株的分离鉴定与生物学特性研究

Isolation,Identification and Biological Characteristics of Mycoplasma synoviae Strain from Henan Province

为了解河南省规模化鸡场滑膜炎的感染情况,采集腱鞘炎、滑膜炎、骨关节炎、气囊炎、心包炎等症状病死鸡的肺脏、心包积液、关节液等病料共159份。通过病原分离培养、16S rRNA基因序列测定比对,共分离鸡滑液囊支原体25株,分离率为15.7%,表明河南省鸡滑膜炎感染率较高。将其中分离自某规模化蛋鸡场的分离株命名为HNMsy1,对HNMsy1进行毒力试验和免疫原性试验,结果显示,HNMsy1具有较强毒力,用该菌株制备的疫苗对鸡滑液囊支原体的攻毒保护率为100%。

In order to understand the synovitis infection in large-scale chicken farms in Henan Province,a total of 159 samples of lung pericardial effusion and articular fluid were collected from dead chickens with symptoms of tenosynovitis,synovitis,osteoarthritis,balloon inflammation,pericarditis,etc.A total of 25 Mycoplasma synoviae strains were identified by pathogen isolation and culture,16S rRNA gene sequencing and blasting.The separation rate was 15.7%,indicating a high infection rate of chicken synovitis in Henan Province.A strain which was isolated from a certain scale egg farm was named HNMsy1.Virulence test and immunogenicity test were performed on HNMsy 1.Results showed that HNMsy 1 had strong virulence to chickens.The challenge protection rate of the vaccine prepared by this strain against Mycoplasma synoviae was 100%.