骨松安激活Runx 2/Osterix途径促进骨质疏松性骨折愈合的机制研究

Study on the mechanism of Gusongan activating runx 2/osterix pathway to promote the healing of osteoporotic fractures

目的初步探索骨松安促进骨质疏松性骨折愈合的机制。方法建立骨质疏松性骨折大鼠模型,采用骨松安进行治疗,分别于7、14、21 d取出骨折端骨痂,通过HE染色观察骨痂生长情况,采用免疫组化、RT-qPCR检测Runx 2、Osterix的表达。结果HE染色结果显示,骨松安治疗可在早期促进骨质疏松性骨折大鼠骨折端软骨细胞增生、成骨细胞成熟分化及编织骨形成。免疫组化、RT-qPCR结果显示,骨质疏松性骨折组大鼠Runx 2与Osterix的表达一直处于较低水平,在骨松安持续治疗下,Runx 2与Osterix的表达得到明显改善。结论骨松安可激活Runx 2/Osterix途径,加速前成骨细胞向成骨细胞分化,促进骨质疏松性骨折大鼠的骨折愈合。

Objective To preliminarily explore the mechanism of Gusongan’s promotion of osteoporotic fracture healing.Methods A rat model of osteoporotic fracture was established and treated with Gusongan.The callus at the fractured end was taken out on 7 days,14 days,and 21 days.The growth of the callus was observed by HE staining.The expression of Runx2 and Osterix were detected by immunohistochemistry and RT-qPCR.Re sults The result of HE staining showed that Gusongan treatment can early promote the proliferation of chondrocytes,the maturation and differentiation of osteoblasts and the formation of braided bone in the fracture end of osteoporotic fracture rats.The result of immunohistochemistry and RT-qPCR showed that the expression of Runx2 and Osterix in the osteoporotic fracture group had been at a lowlevel,and the expression of Runx2 and Osterix had been significantly improved under the continuous treatment of osteoporosis.Conclusion Gusongan can activate the Runx2/Osterix pathway,accelerate the differentiation of pre-osteoblasts into osteoblasts,and promote fracture healing in rats with osteoporotic fractures.