摘要
在烟草中过量表达MYB转录因子LrAN2,能够激活上调花青素生物合成代谢通路,但富含半胱氨酸跨膜结构域蛋白A(CYS)的表达量明显降低。作为植物中一种重要的生物氨基酸,CYS的生物学研究鲜有报道。本研究克隆烟草的CYS基因,其全长为282 bp,编码氨基酸数94个。预测编码的蛋白分子量和等电点分别为24019.84 Da和5.23,并且序列中没有保守区。实验结果显示该蛋白具有亲水性,主要由α-螺旋(19.35%)和无规则卷曲(65.59%)构成,CYS蛋白质不通过跨膜区,在膜外进行作用。系统发育树显示,烟草CYS基因与辣椒CYS基因亲缘关系最近,其次是番茄的HTC基因和WIH2基因。本研究结果克隆并分析CYS基因编码区序列,为CYS基因的功能研究提供参考依据。
Overexpression ofMYB transcription factor LrAN2 in tobacco can activate up-regulation of anthocyanin biosynthetic pathways,but the expression of cysteine-rich transmembrane domain protein A(CYS)is significantly reduced.As an important biological amino acid in plants,the biological research of CYS is rarely reported.In this study,the cloned tobacco CYS gene is 282 bp long and encoding 94 amino acids.The predicted molecular weight and isoelectric point of the encoded protein are 24019.84 Da and 5.23,respectively,and there are no conserved regions in the sequence.The experimental results show that the protein is hydrophilic,mainly composed ofα-helix(19.35%)and random coil(65.59%),CYS protein does not pass through the transmembrane region and acts outside the membrane.Phylogenetic tree shows that the CYS gene in tobacco was closely related to the CYS gene in pepper,followed by HTC gene and WIH2 gene in tomato.The results of this study cloned and analyzed the sequence of the coding region ofCYS gene to provide a reference for the functional study of CYS gene.
作者
罗香怡
高洁铭
刘明慧
曹东
宗渊
刘宝龙
魏乐
Luo Xiangyi;Gao Jieming;Liu Minghui;Cao Dong;Zong Yuan;Liu Baolong;Wei Le(College of Life Sciences,Qinghai Normal University,Xining,810008;College of Agriculture and Animal Husbandry,Qinghai University,Xining,810016;Key Laboratory of Crop Molecular Breeding of Qinghai Province,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810001)
出处
《分子植物育种》
CAS
北大核心
2021年第15期4873-4879,共7页
Molecular Plant Breeding
基金
青海省应用基础研究项目(2018-ZJ-762)
青海省科技成果转化专项-前补助(2018-NK-133)共同资助。