水稻B3转录因子OsL1的生信分析及表达模式

Analysis of Bioinformatics and Expression Pattern of Rice B3 Transcription Factor OsL1

B3转录因子是植物特有的转录因子,与植物花形态建成、激素信号转导、种子生长发育及植物逆境胁迫响应密切相关。前期研究中,利用穗发育芯片筛选到一个OsL1基因。生物信息学分析发现,OsL1基因位于水稻4号染色体,基因全长4262 bp,编码433个氨基酸,蛋白分子量为48.7 kD,序列比对和系统发育分析表明OsL1属于B3家族转录因子,不含信号肽剪切位点和跨膜结构域,亚细胞定位于细胞核。启动子序列分析发现,该基因启动子中含有8个光响应元件及部分激素响应元件。实时PCR分析发现OsL1在水稻根、茎、叶、和穗中均有表达,其中在根和穗发育7期表达量较高,进一步克隆OsL1启动子区域,构建pCAMBIA1301-OsL1-GUS表达载体,遗传转化并鉴定获得转基因植株,The previous study在水稻根、茎、叶、和穗中均检测到了GUS信号,表明OsL1属于组成型表达。以上结果为深入研究OsL1基因在水稻生长发育过程的生物学功能提供了科学依据。

B3 transcription factors are plant-specific transcription factors that are closely related to plant flower morphogenesis,hormone signaling,seed growth and development,and plant stress response to adversity.The previous study screened an OsL1 gene using a spike developmental microarray,and bioinformatics analysis revealed that the OsL1 gene is located on rice chromosome 4,with a full-length of 4262 bp,encoding 433 amino acids,and a protein molecular weight of 48.7 kD.Sequence comparison and phylogenetic analysis indicated that OsL1 belongs to the B3 family of transcription factors,does not contain signal peptide cleavage sites or transmembrane domains,and subcells are located in the nucleus.Sequence analysis of the promoter revealed that the gene contains eight light-responsive elements and some hormone-responsive elements in the promoter.Real-time PCR analysis revealed that OsL1 was expressed in rice roots,stems,leaves,and spikes,with higher expression in root and spike development at stage 7.The OsL1 promoter region was further cloned and the pCAMBIA1301-OsL1-GUS expression vector was constructed,genetically transformed and characterized to obtain transgenic plants,and we detected GUS signals in rice roots,stems,leaves,and spikes,indicating that OsL1 is constitutively expressed.The above results provide a scientific basis for an in-depth study of the biological functions of OsL1 gene in the growth and development of rice.