叶用芥菜晚抽薹性状候选基因预测与表达分析

Prediction and Expression Analysis of Candidate Gene for Late Bolting Traits in Leaf Mustard

本研究在前期的初定位基础上,对主效QTL区段内的基因进行生物信息学分析,发现20个与调控抽薹开花相关的基因。利用RT-qPCR技术分析这些基因在叶用芥菜移栽7 d和移栽75 d后的表达量变化,结果表明,BjuA001637、BjuA009062、BjuA009068、BjuA009109、BjuA009116、BjuA009119、BjuA009123、BjuA009174、BjuA009092、BjuA009108、BjuA009163、BjuA009164、BjuA009205等13个基因在早抽薹亲本MU056抽薹前后无特异性表达,在晚抽薹亲本MN001生长过程中表现为特异性上调表达,因此推测这13个基因为叶用芥菜晚抽薹的候选基因。本研究为进一步开展叶用芥菜晚抽薹的分子机理研究提供理论依据。

In this study,based on the initial positioning in the previous period,20 genes related to bolting and flowering were found by bioinformatics analysis.Expression changes of these genes were analyzed by RT-qPCR technology after transplanting leaves with mustard for 7 and 75 d,the results showed that BjuA001637,BjuA009062,BjuA009068,BjuA009109,BjuA009116,BjuA009119,BjuA009123,BjuA009174,BjuA009092,BjuA009108,BjuA009163,BjuA009164 and BjuA009205 had no specific expression before and after bolting of early bolting parent MU056,but showed specific up-regulation expression during the growth of late bolting parent MN001.Therefore,these 13 genes are speculated as candidate genes for late bolting of leaf mustard.This study provides a theoretical basis for further research on the molecular mechanism of late bolting of leaf mustard.