大白菜自交系背景选择InDel标记与重要农艺性状的关联分析

Association Analysis Between Background Selection InDel Markers and Important Agronomic Traits in Inbred Lines of Chinese Cabbage

InDel背景选择标记有利于大白菜育种亲本的高效选配。以31份不同类型大白菜自交系为材料进行农艺性状变异分析、InDel标记遗传背景和群体结构分析,并将多态性标记与农艺性状相关联。结果显示,31个农艺性状的变异系数变幅为15.97%~47.63%,平均为32.58%,表明供试群体表型变异丰富;从177对InDel引物中筛选出158对多态性引物,在31份材料中共检测出701个多态性等位变异位点,平均每对引物检测到等位变异位点4.418个,变幅为1~11个;标记位点的多态性信息含量(PIC)变幅为0.612~0.963,平均为0.843;有效等位基因变异数(Ne)变幅为1.656~3.985,平均为2.640;供试材料遗传相似系数(GS)在0.575~0.910之间,变化幅度较大,说明群体的遗传背景具有较大差异。群体结构分析将供试材料划分为3个亚群;关联分析发现分布于8个连锁群的22个InDel标记与供试材料的12个农艺性状(开展度、全株质量、球叶数、外叶数、叶片宽、叶片长、叶球高、叶球宽、叶球质量、中肋长、中心柱长和株高)相关。

The InDel marker for genetic background selection is beneficial to the efficient selection of Chinese cabbage breeding parents.Taking 31 inbred lines of Chinese cabbage with different ecological types as materials,InDel genetic background and association of agronomic traits were analyzed in this study.The results showed that the coefficient of variation of 31 agronomic traits varied from 15.97%to 47.63%,with an average of 32.58%,indicating that the phenotypic diversity of tested population was rich.Finally,158 polymorphic primer pairs were selected from 177 InDel primer pairs.A total of 701 polymorphic alleles were detected in 31 accessions of Chinese cabbage,with an average of 4.418 alleles per primer pair,ranging from 1 to 11.The polymorphic information content(PIC)of the marker loci ranged from 0.612 to 0.963,with an average of 0.843,and the number of effective alleles(N e)ranged from 1.656 to 3.985,averagely 2.640.The genetic similarity coefficient(GS)of tested materials of Chinese cabbage varied greatly from 0.575 to 0.910,indicating that the genetic background of the population was quite different.According to population structure analysis,tested materials were divided into three subgroups.Association analysis showed that 22 InDel molecular markers distributed in eight linkage groups related to 12 agronomic traits,including expansion spreading,whole plant weight,head leaf number,outer leaf number,leaf width,leaf length,leaf head height,leaf head width,leaf head weight,middle rib length,central column length and plant height.