培植牛黄中胆红素的测定及含量考察研究

Quantitation and content investigation of bilirubin in cultured cow-bezoar

建立培植牛黄中胆红素的定量测定方法,并对15批培植牛黄样品中胆红素成分的含量进行测定和考察。使用10%草酸溶液对培植牛黄进行处理,使其中的非游离型胆红素转化成游离型胆红素,然后通过二氯甲烷进行液液萃取,所得供试品溶液由Agilent TC-C18色谱柱(4.6 mm×250 mm, 5μm),经乙腈和1%冰醋酸水溶液(95∶5)作为流动相,在流速为1.0 mL·min^(-1)进行洗脱,450 nm下进行测定。结果15批培植牛黄中胆红素为21.9%~41.7%,平均含胆红素32.4%。该研究方法准确可靠,适用于培植牛黄中胆红素成分的测定及其质量评价及控制。

A determination method for bilirubin in cultured cow-bezoar was developed in this study, with which the bilirubin in 15 batches of samples was quantified. The samples were first processed with 10% oxalic acid solution for the conversion of bilirubin from conjugated to unconjugated, followed by the extraction with dichloromethane. Then the obtained sample solutions were analyzed at 450 nm by HPLC[chromatographic column: Agilent TC-C18(4.6 mm × 250 mm, 5 μm);mobile phase: acetonitrile and 1% glacial acetic acid aqueous solution(95∶5);flow rate: 1.0 mL·min^(-1)]. The bilirubin content in the 15 batches of cultured cow-bezoar was ranged from 21.9% to 41.7% with the average of 32.4%. The proposed method is accurate and reliable, thus making it suitable for the quantitation of bilirubin in cultured cow-bezoar and its quality assessment and control.