瑞巴派特在大鼠痛风性关节炎急性发作中的作用

Effect of rebamipide on the acute gouty arthritis in rats induced by monosodium urate crystals

目的:了解瑞巴派特(rebamipide)在预防尿酸盐晶体诱导的大鼠痛风性关节炎急性发作中的作用。方法:42只雄性大鼠按随机表法分为3组(n=14),以尿酸盐晶体制备大鼠急性痛风性踝关节炎模型,A组口服瑞巴派特治疗,B组口服秋水仙碱治疗,C组口服安慰剂治疗,观察各组踝关节的临床表现及病理变化,检测大鼠血清中白细胞介素(interleukin,IL)-1β、IL-6、IL-10、肿瘤坏死因子(tumor necrosis factor,TNF)-α水平。结果:C组大鼠关节炎的临床评分、肿胀指数在24 h达到最大值,后逐渐下降,72 h基本恢复正常。造模24 h后,C组的临床评分明显高于A组和B组[2(1~3)vs.0(0~1)vs.1(0~2)],差异有统计学意义(P=0.008);C组的肿胀指数明显高于A组和B组[0.36(0.16~0.52)vs.0.11(0~0.20)vs.0.12(0~0.16)],差异有统计学意义(P=0.005);组织学上,C组的滑膜组织中有大量中性粒细胞浸润[5分量表评分为4(2~4)],A组[1(0~2)]与B组[1(0~2)]未见明显炎性细胞浸润,差异有统计学意义(P<0.001)。造模24 h后,C组大鼠血清中IL-1β、IL-6、IL-10和TNF-α水平明显高于A组和B组[IL-1β:(41.86±5.72)vs.(27.35±7.47)vs.(27.76±5.28)ng/L;IL-6:(1575.55±167.11)vs.(963.53±90.22)vs.(964.08±99.31)ng/L;IL-10:(37.96±3.76)vs.(21.68±4.83)vs.(16.20±2.49)ng/L;TNF-α:(21.32±1.34)vs.(15.82±2.54)vs.(17.35±7.47)μg/L],差异均有统计学意义(P<0.001)。结论:瑞巴派特在大鼠痛风模型中对痛风性关节炎急性发作起预防保护作用。

Objective:To investigate the role of rebamipide in the treatment of acute gout arthritis rats induced by monosodium urate(MSU)crystal.Methods:Forty-two male rats were randomly divided into three groups(n=14).Group A was treated with oral rebamipide,group B with oral colchicine,and group C with oral placebo.The rats were monitored for the induction of arthritis with clinical manifestations and pathological changes,and the levels of interleukin(IL)-1β、IL-6、IL-10,and tumor necrosis factor(TNF)-αin serum were measured.Results:In group C,the clinical score and swelling index reached the maximum in 24 h,and then gradually decreased to 72 h.After 24 h of model induced,the clinical scores in group C were significantly higher than those in group A and group B[2(1-3)vs.0(0-1)vs.1(0-2),P<0.01],the swelling indexes in group C were significantly higher than those in group A and group B[0.36(0.16-0.52)vs.0.11(0-0.20)vs.0.12(0-0.16),P<0.01].Histologically,after 24 h of model induced,there was a large number of neutrophil infiltration in the synovium of group C[scale score:4(2-4)],and there was no significant inflammatory cell infiltration in group A[1(0-2)]and group B[1(0-2)],the difference was statistically significant(P<0.001).After 24 h of model induced,the levels of IL-1β,IL-6,IL-10,and TNF-αin serum of group C were significantly higher than those in group A and B[IL-1β:(41.86±5.72)vs.(27.35±7.47)vs.(27.76±5.28)ng/L,IL-6:(1575.55±167.11)vs.(963.53±90.22)vs.(964.08±99.31)ng/L,IL-10:(37.96±3.76)vs.(21.68±4.83)vs.(16.20±2.49)ng/L,TNF-α:(21.32±1.34)vs.(15.82±2.54)vs.(17.35±7.47)μg/L,P<0.001].Conclusion:Rebamipide has a protective effect on acute gout arthritis rats induced by MUS crystals.