一株罗非鱼源无乳链球菌的分离鉴定及耐药性分析

Isolation,identification and drug resistance analysis of a Streptococcus agalactiae strain from tilapia

从海南省三亚市崖州区养殖的罗非鱼中分离得到一株优势病原菌,命名为HH-0708.经由16S rDNA序列测定、菌落形态观察、生理生化试验,HH-0708被鉴定为无乳链球菌。HH-0708体外药敏结果显示:其对青霉素、头孢呋辛、多西环素等11种抗生素高度敏感,而对四环素、庆大霉素等9种抗生素表现出较强的耐药性,对链霉素、红霉素等其他5种抗生素,表现为中度敏感。

A pathogen named HH-0708 was isolated from tilapia cultured in Yazhou District,Sanya City,Hainan Province.The HH-0708 strain was identified as Streptococcus agalactiae by a series of tests which included morphological observation,16S rDNA sequencing,physiological and biochemical identification.The antibiotic sensitivity analysis indicated that HH-0708 was extremely susceptible to 11 kinds of drugs including penicillin,ceftriaxone,doxycycline,etc,and medium sensitive to streptomycin,erythromycin and other three drugs.While HH-0708 was resistant to nine drugs,such as tetracycline,gentamicin,etc.