钙化性心瓣膜病合并肺动脉高压病人血清miR-222、白细胞介素-6、脑钠肽水平分析

Analysis of serum levels of miR-222,interleukin-6 and brain natriuretic peptide in patients with calcified valvular heart disease and pulmonaryarterial hypertension

目的探讨钙化性心瓣膜病(calcified valvular heart disease,CVHD)合并肺动脉高压(pulmonary arterial hypertension,PH)病人微小RNA-222(miR-222)、IL-6、hs-CRP、N末端脑钠肽前体(N-terminal pro-brain natriuretic peptide,NT-proBNP)的水平及其临床意义。方法选择2018年8月至2019年5月在我院住院的CVHD病人158例,分为单纯CVHD组(n=86)及CVHD合并PH组(n=72),另选30名健康体检者作为对照组。比较3组血清miR-222、IL-6、NT-proBNP、hs-CRP的水平差异,并分析miR-222与IL-6、NT-proBNP、hs-CRP的相关性。结果CVHD病人血清miR-222、IL-6、NT-proBNP、hs-CRP的水平明显升高,尤其是合并PH的CVHD病人升高更明显(P<0.05或P<0.01)。相关性分析显示,所有CVHD病人IL-6、NT-proBNP、hs-CRP水平与miR-222水平呈正相关(r=0.507、0.390、0.509,均P<0.01)。结论血清miR-222及IL-6等介导的炎性反应参与了CVHD及其合并PH的进展及预后。

Objective To explore the serum levels of microRNA-222(miR-222),interleukin-6(IL-6),high-sensitivity C-reactive protein(hs-CRP)and N-terminal pro-brain natriuretic peptide(NT-proBNP)and their clinical value in the patients with calcified valvular heart disease(CVHD)and pulmonaryarterial hypertension(PH).Methods A total of 158 patients with CVHD were divided into CVHD group(n=86)and CVHD combined with PH group(n=72).Thirty healthy people were selected as the control group.The serum levels of miR-222,IL-6,NT-proBNP and hs-CRP were detected and compared among the three groups,and the correlation between miR-222 and IL-6,NT-proBNP,hs-CRP was analyzed.Results The serum levels of miR-222,IL-6,NT-proBNP and hs-CRP in CVHD group increased significantly,especially in CVHD combined with PH group(P<0.05 or P<0.01).In the patients with CVHD,the serum levels of IL-6,NT-proBNP,hs-CRP,miR-222 were all positively correlated with the level of miR-222(r=0.507,0.390,0.509,P<0.01).Conclusions Serum inflammatory factors such as miR-222 and IL-6 are involved in the occurrence and progression of CVHD and the development of PH.