The Application of Next‑Generation Sequencing to Validate D12S391 Microvariation

Objective:In a paternity case,the D12S391 locus was reported as a mismatch.To confirm the existence of mutations and mutations come from father or mother.Methods:STR and next-generation sequencing technology were used to validate the sequence.Results:NGS showed the loss of one adenine between the 19.3 allele of the child and allele 20 of the mother.Conclusion:The NGS can be applied in the paternity to validate the mutation.