膜型基质金属蛋白酶-2(MT2-MMP)在NSCLC组织中的表达及临床意义研究

Study on the expression and clinical significance of membrane carbonate proteinase-2(MT2-MMP)in NSCLC tissues

目的:探讨MT2-MMP在NSCLC组织中的表达情况,与NSCLC临床病理特征之间的关系及MT2-MMP对NSCLC预后的价值。方法:收集2015年01月至2018年06月于我院外科接受手术治疗的NSCLC患者的NSCLC组织及其癌旁正常肺组织各89例。采用免疫组化SP法检测NSCLC组织及其癌旁正常肺组织中MT2-MMP的表达水平。统计分析MT2-MMP蛋白与NSCLC患者临床病理特征、预后之间的相关性。结果:MT2-MMP在NSCLC组织中高表达62.9%(56/89)显著高于癌旁正常组织5.6%(5/89),差异有统计学意义(P<0.001)。MT2-MMP的表达与肿瘤分化程度及淋巴结转移有关(P<0.05),与患者性别、年龄、吸烟史、肿瘤直径、组织学类型、临床TNM分期无关(P>0.05)。生存曲线分析提示MT2-MMP高表达组生存率显著低于低表达组(χ^(2)=4.536,P=0.033)。Cox单因素分析结果显示:吸烟、TNM分期Ⅲ-Ⅳ期、低中分化、淋巴结转移、MT2-MMP高表达是影响NSCLC患者预后的危险因素(P<0.05);多因素分析结果显示:TNM分期Ⅲ-Ⅳ期、低中分化、淋巴结转移、MT2-MMP高表达是NSCLC患者预后的独立危险因素(P<0.05)。结论:MT2-MMP高表达与NSCLC患者的不良预后有关,提示MT2-MMP有可能作为NSCLC患者的预后标志物及临床检测或联合检测的指标,提高临床诊断率。

Objective:To investigate the expression of MT2-MMP in NSCLC,the relationship between MT2-MMP and clinicopathological features of NSCLC,and the prognostic value of MT2-MMP in NSCLC.Methods:From Jan.2015 to Jun.2018,the NSCLC tissues of patients with NSCLC and their adjacent normal lung tissues were collected from 89 patients who underwent surgery in our hospital.Immunohistochemical SP method was used to detect the expression of MT2-MMP in NSCLC and its adjacent normal lung tissues.Statistical analysis of the correlation between MT2-MMP protein and clinical pathological features and prognosis in patients with NSCLC.Results:The high expression of MT2-MMP in NSCLC tissues was 62.9%(56/89),which was significantly higher than that in adjacent normal tissues[5.6%(5/89)].The difference was statistically significant(P<0.001).Statistical analysis showed that the expression of MT2-MMP was related to tumor differentiation and lymph node metastasis(P<0.05),and had nothing to do with patient gender,age,smoking history,tumor diameter,histological type,and clinical TNM stage(P>0.05).The survival curve analysis showed that the survival rate of the MT2-MMP high expression group was significantly lower than that of the low expression group(χ^(2)=4.536,P=0.033).Cox univariate analysis showed that smoking,TNM stageⅢtoⅣ,poor to moderate differentiation,lymph node metastasis,and high expression of MT2-MMP were risk factors affecting the prognosis of patients with NSCLC(P<0.05).Multivariate analysis showed that TNM stageⅢ-Ⅳ,poor to moderate differentiation,lymph node metastasis,and high expression of MT2-MMP were independent risk factors for prognosis in patients with NSCLC(P<0.05).Conclusion:The high expression of MT2-MMP is related to the poor prognosis of NSCLC patients,suggesting that MT2-MMP may be used as a prognostic marker and an indicator of clinical detection or combined detection in patients with NSCLC to improve the clinical diagnosis rate.