山葡萄花青素对慢性酒精中毒小鼠认知障碍的改善作用

Effects of anthocyanins from Vitis amurensis on cognitive impairment in mice with chronic alcoholism

目的:研究山葡萄花青素对慢性酒精中毒介导认知障碍的改善作用。方法:将小鼠分为空白组、模型组、山葡萄花青素低剂量组和山葡萄花青素高剂量组,建立慢性酒精中毒模型并进行山葡萄花青素灌胃干预,测定Morris水迷宫和跳台试验、抗氧化应激和胆碱能系统相关指标,并对小鼠海马体进行切片观察。结果:山葡萄花青素干预后水迷宫试验逃避潜伏期缩短了41.38%、游泳总游程缩短了28.53%、首次登台时间缩短了27.00%、穿台次数增加至(3.00±2.55)次、第Ⅳ象限停留时间和路程百分比分别增加了21.13%和24.24%,跳台试验跳台潜伏期延长了26.39%和26.61%、错误次数减少至(3.38±0.92)次和(2.63±0.74)次;增加了血清和海马体中超氧化物歧化酶(分别增加8.62%和6.80%)、过氧化氢酶(25.34%和17.39%)、谷胱甘肽(28.14%和3.57%)、谷胱甘肽过氧化物酶(16.14%和25.85%)水平,丙二醛含量分别降低了21.24%和27.70%;降低了海马体中乙酰胆碱酯酶活性,增加了乙酰胆碱含量。结论:山葡萄花青素能够提高慢性酒精中毒小鼠抗氧化应激能力,有效对抗胆碱能损伤,促进神经传递并改善认知障碍。

Objective:To study the effect of Vitis amurensis.anthocyanins on cognitive impairment induced by chronic alcoholism.Methods:The mice were divided into blank group,model group,low-dose and high-dose groups treated by anthocyanins from V.amurensis.The model of chronic alcoholism was established and intervened with anthocyanin from V.amurensis.Morris water maze and step-down test,antioxidant stress and cholinergic system related indexes were measured,and the hippocampal slices were observed.Results:After anthocyanins intervention,the escape latency of water maze test was shortened by 41.38%,the total swimming distance was shortened by 28.53%,the first stage time was shortened by 27.00%,the times of crossing the stage was increased to(3.00±2.55)times,the residence time in the fourth quadrant was increased by 21.13%,the percentage of distance in the fourth quadrant was increased to 24.24%,the platform jumping latency was prolonged by 26.39%and 26.61%,the number of errors was reduced to(3.38±0.92)and(2.63±0.74)times.Moreover,the levels of total superoxide dismutase(8.62%and 6.80%),catalase(25.34%and 17.39%),glutathione(28.14%and 3.57%),glutathione peroxidase(16.14%and 25.85%)were increased and malondialdehyde was decreased by 21.24%and 27.70%in serum and hippocampus.Acetylcholinesterase activity in hippocampus was decreased and the acetylcholine content was increased.Conclusion:Anthocyanins from V.amurensis could improve the ability of anti-oxidative stress in mice with chronic alcoholism,resist cholinergic injury effectively,promote neurotransmission and cognitive impairment.