贵州狐臭柴不同部位提取物及其抗氧化活性

Flavonoid constituents and antioxidant activities of crude ethanolic extracts from different tissues of Premna puberula Pamb.in Guizhou

目的:丰富天然抗氧化剂的来源。方法:以贵州地区的狐臭柴(Premna puberula Pamb.)为原料,超声提取并检测了根、茎、叶中总酚和总黄酮含量,比较了根、茎、叶中柚皮素、芹菜素、芦丁、槲皮素4种黄酮类成分含量,测定了提取物对DPPH自由基和ABTS+自由基的清除能力。结果:狐臭柴根、茎、叶中总酚含量分别为3.26,9.58,30.05 mg/g,总黄酮含量分别为0.22,0.58,6.54 mg/g,芦丁和芹菜素含量依次为茎>根>叶。各组织提取物的抗氧化活性均随浓度的升高而增强,与总酚、总黄酮含量呈正相关,其中叶提取物的抗氧化活性最强,当叶质量浓度为0.1 mg/mL时,DPPH自由基和ABTS自由基的清除率分别为82.5%,81.9%,与维生素C的总还原力接近。结论:狐臭柴具有良好的抗氧化活性。

Objective:To enrich natural sources of antioxidants.Methods:The scavenging ability of DPPH and ABTS+of extracts from Guizhou Premna puberula Pamb.was detected by ultrasonic extraction of total phenols and total flavonoids from roots,stems and leaves.Results:All the tissues had certain antioxidant activity and the antioxidant activity of leaves was the strongest,which was positively correlated with the contents of total phenols and total flavonoids.When the mass concentration was 0.1 mg/mL,the DPPH radical scavenging rate of leaves could reach 82.5%,which was close to the total reducing power of V C.Conclusion:It indicated that P.puberula has good antioxidant activity.